【作者】 于景丽；

【导师】 张小平； 李登煜； 陈强；

【作者基本信息】 四川农业大学 ， 土壤学， 2003， 硕士

【摘要】 本研究以缺铁和缺钼紫色土为供试土壤做盆栽实验，选用三株慢生型花生根瘤菌Spr3-7、Spr4-5、Spr3-5及gusA和celB标记的菌株A3-7、A4-5、A3-5，B3-7、B4-5、B3-5接种天府9号花生。通过标记根瘤菌形成的根瘤能与检测试剂产生颜色反应的特征，检测施铁钼及施不同浓度的铁钼对花生-根瘤菌共生有效性及竞争性的影响。结果表明： 缺铁石灰性紫色土上，喷铁、接种、喷铁+接种均能促进花生与根瘤菌的共生固氮效应和竞争结瘤能力，其作用效果依次是：喷铁+接种＞接种＞喷铁。与喷施0.3％的硫酸亚铁相比，喷施0.2％的铁更能增加植株干重、瘤重、总瘤数、叶绿素含量、全氮含量。叶绿素作为指示共生固氮效应的重要指标，与全氮含量，花生产量，植株干重，瘤重存在极显著的相关性，相关系数分别为：0.806、0.795、0.637、0.585。 通过gusA和celB标记方法检测到喷施0.2％的铁更能促进接种根瘤菌的竞争结瘤能力，供试菌株的竞争性从强到弱的顺序是Spr4-5＞Spr3-7＞Spr3-5。两种标记方法检测的结果基本一致，两种标记根瘤菌的平均占瘤率分别为79.64％、75.62％、74.41％。 缺钼酸性紫色土上施钼+接种菌的效果最好，接种次之，施钼最差。单施钼时，用0.4％的钼酸铵拌种效果好；接种时，0.2％的效果好。供试菌株的竞争性从强到弱的顺序是Spr4-5＞Spr3-7＞Spr3-5。更多还原

【Abstract】 In this research, two types of soil which were lack of iron and molybdenum were used in pot experiment.Three strains of Bradyrhizobium Spr3-7,Spr4-5,Spr3-5 and their corresponding gene marker strains gusA3-7,gusA 4-5, gusA 3-5,celB3-7, celB 4-5 celB 3-5 with gusA and celB gene were selected to inoculate TianFu 9 peanut. Root nodule formed by gene marker strains had the ability to produce special color when reacting with detecting reagent. We used this characteristic to detect the effect of applying iron and molybdenum and their different level of iron and molybdenum on the symbiotic effectiveness and competitiveness of peaiait-Bradyrhizobium strains.Results showed that in calcareous purple soil because of available iron deficient, spraying iron, inoculatation, spraying iron + inoculation could all promote symbiotic nitrogen-fixtion effect of peaimt-Bradyrhizobium, their effect from higher to lower were spraying iron + inoculation> inoculatation > iron .Spraying 0.2% FeSO4.H2O solution could increase shoot dry weight, nodule weight ,total nitrogen content, total chlorophyll content, much more than 0.3% FeSO4.H2O solution. Correlation coefficient between total chlorophyll and total nitrogen, peanut yields, shoot dry weight, nodule weight were respectively 0.806, 0.637, 0.795 , 0.585.Spraying 0.2% FeSO4.H2O solution could promote competitive ability of peanut and inoculated Bradyrhizobium strains better than that of 0.3%, their competiveness in turn was Spr4-5>Spr3-7>Sp3-5. Ratio of occupancy detected by two gene marker techniques had no significant difference, average numerical value of them was respectively 79.46%, 75.62%, 74.41%.In acid purple soil because of available molybdenum deficient, effect of applying Mo + inoculation was better than inoculation , and inoculation was better than applying Mo. Effect of 0.4% NH4.MoO3.xH2O solution was better than 0.2% when they were applied alone ,while 0.2% NH4.MoO3.xH2O solution was better than 0.4% in inoculating Bradyrhizobium strains. Competiveness of Bradyrhizobium tested in turn was Spr4-5>Spr3-7>Sp3-5 .更多还原