【作者】 孙茂红；

【导师】 乔健；

【作者基本信息】 中国农业大学 ， 基础兽医学， 2003， 硕士

【摘要】 本试验旨在探讨两种钙拮抗剂(Calcium Antagonists，CA)对肉鸡肺动脉高压(Pulmonary Hypertension，PH)和腹水综合征(Ascites Syndrome，AS)的影响。从而较深层的揭示肉鸡肺动脉高压的形成机理，为预防和治疗肉鸡腹水综合征提供理论依据。200只AA商品代肉公仔鸡常规饲养，15日龄随机分为四组。50只在常温(19—24℃)环境下饲养为常温组，50只在低温(8-11℃)环境下饲养为低温组，50只在低温(8-11℃)环境下饲养并饲喂硝苯地平(Nifedipine，Nif)为硝苯地平组，50只在低温(8-11℃)环境下饲养并饲喂维拉帕米(Verapamil，Ver)为维拉帕米组。饲养过程中，分别在28、36和44日龄随机选取10只，采用右心导管法测定肺动脉收缩压(Pulmonary Artery contract Pressure，PACP)、肺动脉舒张压(Pulmonary Artery Stretch Pressure，PASP)、平均肺动脉压(mean Pulmonary Artery Pressure，mPAP)、右心室收缩压(Right Ventricular Systolic Pressure，RVSP)、右心室舒张压(Right Ventricular Diastolic Pressure，RVDP)、右心室内压的最大变化速率(±dp/dtmax)(RV)、红细胞压积(Packed Cell Volume，PCV)、和腹水心脏指数(Ascites Heart Index，AHI)，最后统计腹水发生率。研究结果如下： 1．在28和36日龄，低温组mPAP为3.44±0.7kPa和3.4±1.3kPa，常温组为2.2±0.7kPa和1.8±0.1kPa，低温组mPAP明显高于常温组(P＜0.05)；44日龄，低温组mPAP是3.7±0.7kPa，常温组是2.7±1.2kPa，低温组mPAP极明显高于常温组(P＜0.01)。 2．在28和36日龄，硝苯地平与低温组比降低mPAP(P＞0.05)；44日龄，低温组为3.7±0.7kPa，硝苯地平组为2.4±0.2kPa，硝苯地平组mPAP极明显低于低温组(P＜0.01)；硝苯地平降低AS的发生率(P＞0.05)。 3．在28和36日龄低温组mPAP为3.4±0.7kPa和3.4±1.3kPa，维拉帕米组mPAP为1.8±0.8kPa和1.3±0.3kPa，与低温组比维拉帕米明显降低mPAP(P＜0.05)；44日龄维拉帕米组mPAP极明显低于低温组(P＜0.01)。维拉帕米明显降低低温诱发AS的发生率(P＜0.05)。 本试验结果显示，维拉帕米和硝苯地平降低了低温诱发肺动脉高压和AS的发生率，初步证实钙信号参与了肺动脉高压的形成，对AS的发生发展具重要的作用。更多还原

【Abstract】 The experiment was to evaluate the effects of Calcium Antagonists on Pulmonary hypertension (PH) and the mechanism of the Calcium Signal in PH in broilers. 200 AA male commercial broiler chickens were randomly divided into four groups on 15-day-old, normal ambient temperature group (19-24℃) (NT); three low temperature groups (9-11℃), including blank group (LT), Nifedipine group (p.o.)(Nif), Verapamil group (p.o.)(Ver), 50 each group. 10 birds were selected from each group at the age of 28, 36 and 48 days for measuring the Pulmonary Artery Contract Pressure (PACP), Pulmonary Artery Stretch Pressure (PASP), Right Ventricular Systolic Pressure (RVSP), Right Ventricular Diastolic Pressure (RVDP), Maximum change ratio of Right Intraventricular Pressure (RV±dp/dt max), the Packed Cell Volume (PCV), the Ascites Heart Index (AHI) and the incidence of AS. The results were as follows:1. The mPAP of LT on 28 and 36 days old(3.4±0.7kPa, 3.4±1.3kPa) were higher than that of NT ( 2.2±0.7kPa, 1.7 ±0.1kPa) (P<0.05), respectively;The mPAP of LT in 44 days old(3.7 ±0. kPa) was Significantly higher than that of NT(2.7 ±1.2kPa) (P<0.01).2. The mPAP of Nif were decrease than that of LT on 28 and 36 days old(P>0.05); The mPAP of Nif 44days old(3.7±0.7kPa)was Significantly lower than that of LT(2.4±0.2kPa) (P<0.01). Incidence of AS of Nif was low than that of LT (P>0.05).3. The mPAP of Ver on 28 and 36 days old(1.8±0.8kP and 1.3±0.3kPa)was lower than that of LT(3.4±0.7kPa and 3.4±1.3kPa), respectively(P<0.05). The mPAP of Ver was Significantly lower than that of LT on 44 days old (P<0.01). Incidence of AS of Ver was lower than that of LT (P<0.05).Ver and Nif decrease mPAP and incidence of AS, We can get a conclusion that calcium signal play an important action in the development of PH and occurrance of AS.更多还原