【作者】 王锂韫；

【导师】 贺银凤；

【作者基本信息】 内蒙古农业大学 ， 农产品加工及贮藏工程， 2003， 硕士

【摘要】 本项研究对象为分离自内蒙古锡林郭勒盟地区牧民家庭采集的10份酸马奶酒样中的47株乳酸菌，其中乳杆菌属26株，乳酸球菌21株，主要进行了抑菌特性的研究。结果表明，47株乳酸菌的发酵上清液和菌悬液均不能抑制Staphylococcus aureus和Escherichia coli；26株乳杆菌中，有10株乳杆菌仅有菌悬液有抑制指示菌活性，其中6株乳杆菌菌悬液有抑制Listeria monocytogenes和Bacillus subtills的活性，4株乳杆菌菌悬液仅有抑制Listeria monocytogenes的作用。21株乳酸球菌中，9株菌对指示菌有抑菌活性，其中2株菌仅有菌悬液对指示菌有抑制作用，而7株菌不仅菌悬液，发酵上清液也具有抑制指示菌活性，而且Enterococcus faecalis占多数为6株。从其中筛选出两株抑菌活性最强的菌株3-3-2和4-3-2，进行影响抑菌活性物产生等因素以及细菌素粗提，粗提物理化特性及抑菌谱的研究。研究结果发现，菌株3-3-2和4-3-2均在指数生长期开始后，抑菌活性迅速增加，且耐受pH范围较宽。粗提后的抑菌活性物是一类抑菌谱较窄，但具有广谱抑菌活性的分子量大于50KDa的蛋白类物质，对热敏感，耐受pH范围宽，耐受一些表面活性剂，菌株3-3-2发酵液的粗提物还耐受一些蛋白酶和α-淀粉酶。更多还原

【Abstract】 In this study, the antibacterial characterization of forty-seven strains of lactic acid bacteria which isolated from ten samples of koumiss collected from the heardsman’s home in Inner Mongolia-XiLinGuoLe region were studied.The total of forty-seven strains of lactic acid bacteria were not screened for antagonistic activities to Staphylococcus aureus and Escherichia coli.The bodies of only ten Lactobacilli were shown to inhibit the growth of indicator organism-Listeria monocytogenes or Bacillus subtilis among twenty-six Lactobacilli.Nine Lactocilli also exhibited inhibitory activity against indicator organism.Among them there are seven strains which not only the supernatants but also the bodies exhibited inhibitory activity against indicator organism- Listeria monocytogenes or Bacillus subtilis and six of seven Lactocilli were Enterococcus faecalis.Two of the seven Lactocilli,3-3-2 and 4-3-2,which have higher inhibitory activity were chosen for further study.There was a rapid increase of bacteriocin activity after the start of the exponential phase.The maximal level did not decline over thirty hours coinciding with the low pH reached at the time.The antibacterial substances extracted from cell culture liquor were proteins,which activity spectrums was narrow.The activity were not changed more under a wide pH range,but appeared to be heat labile.Some surfactants had no effect on the inhibitory activity. The antibacterial substances of 3-3-2 did not sensitivity to the proteolytic and a -amylase.更多还原