【作者】 张志雄；

【导师】 周长忍；

【作者基本信息】 暨南大学 ， 生物医学工程， 2003， 硕士

【摘要】 本实验的目的是为了制备一种新型壳聚糖衍生物作为组织工程角膜支架材料。 壳聚糖是甲壳素的脱乙酰产物，是储量极为丰富的天然高分子，广泛存在与蟹壳虾壳。壳聚糖由于其良好的生物相容性而受到广泛的重视。壳聚糖能被酶所降解，且降解产物无毒，无免疫源性。甲壳素以及壳聚糖由于良好的生物相容性可以用作伤口包扎，止血海绵等。 而聚乙烯吡咯烷酮是一种无毒的水溶性高分子，有良好的溶解性，化学稳定性以及成膜性。 壳聚糖分子上有许多可反应的基团，可以接枝改性以改善壳聚糖的加工性能。本论文采用西佛碱方法用聚乙烯吡咯烷酮接枝壳聚糖，以期获得性能更加优越的组织工程支架材料。 通过角膜细胞培养实验证明，制备的新型壳聚糖衍生物材料具有良好的生物相容性，是一种很好的角膜组织工程支架材料。更多还原

【Abstract】 This experiment aimed to confirm whether man-made grafted chitosan scaffold is appropriate scaffold for keratocyte culture of tissue engineering.Chitosan is partially N-deacetylated chitin, and chitin is a linear homopolymer of 1,4 β-linked N-acetyl-d-glucosamine. a natural biopolymer available in plenty which is obtained from the cuticle of the marine crustaceans such as crabs and shrimps .Chitosan have neen widely investigated for the last two decades on its biomedical applications.since chitosan can be hydrolyzed by lysozyme,it is also one of the biodegradble polymers in narure.furthermore the degraded products of chitosan are nontoxic,nonimmunogenic and noncarclnogenic. Chitin and chitosan have been tried out as drug carriers because of their biocompatibility and biodegradability. Chitosan and some of its complexes have been studied for use in a number of biomedical applications .Both chitin and chitosan have many useful and advantageous biological properties in the application as a wound dressing, namely biocompatibility, biodegradability, hemostatic activity, anti-infectional activity and property to accelerate wound-healingPVP is a kind of nontoxic and water-solubility macromoleculewhich has some excellent properts such as solubility, chemical stability, membrane formation and so onSince chitosan, chemically, has both reactive amino and hydroxyl groups that can be chemical modi.cations and, physically, is relatively easy to manipulate different pore structures , it has a high potential in applications of tissue engineering. We modified the chitosan by grafting PVP onto it to get a new biomaterials with better biocompatibilityMethods for estimating the degree of substitution by elemental analysis were carried out.The biocompatibility of such grafted chitosan material was evaluated preliminarily in vitro by the culture of keratocyte. Results proved that chitosan is a promising biomaterials for tissue engineering.更多还原