【作者】 陈铎葆；

【导师】 陈志武； 赵维中；

【作者基本信息】 安徽医科大学 ， 药理学， 2002， 硕士

【摘要】 目的：观察红花F_α2对实验性心肌缺血模型、血流动力学及冠脉流量的影响，并探讨其与对抗氧自由基的关系。 方法： 1．采用结扎左冠状动脉前降支制备犬心肌缺血模型，随机分为对照组、红花F_α2(5mg、10mg和40mg·kg^-1 ⅳ)三个剂量组，以及复方丹参（SM）组(0.8g·kg^-1 ⅳ)。分别描记结扎前、结扎后5min、15min、30min、1h、2h、3h及4h的心电图改变。取心脏用NBT染色液染色心肌片，分别称取全心重量、缺血区重量、坏死区重，计算其各占全心重的百分率。采用在位心脏测定冠脉流量法，分别观测给药前，给药后15min，30min，60min的冠脉流量和心肌耗氧量。 2．采用异丙肾上腺素(10mg·kg^-1)制备大鼠急性心肌缺血模型，随机分为对照组、红花F_α2(5mg、20mg和40mg·kg^-1 ⅳ)，行心导管插管术，观察血流动力学指标变化（MBP、LVSP、LVEDP、±dp/dtmax，等）。实验结束后，取适量体循环血清和心肌组织，分别观测MDA含量和SOD的活性变化。 结果： （1）红花F_α2(5mg·kg^-1)组对心肌缺血犬心率有明显减慢作用（P＜0.05），红花F_α2(40mg·kg^-1)组和红花F_α2(10mg·kg^-1)组对心率影响较小。（2）红花F_α2(5mg、10mg·kg^-1)组，SM（5mg·kg女徽广科人学砚1：学位论义－）组对急性心肌缺血大引起的 ST抬高有明显抑制作用（P＜0．05或 k 0．01），且红花 F。。（5 m s·kg－），S M（0．8 s·kg－‘）组间 S T段改变有显著差异（P＜0．05）。（3）急性心肌缺血大红花F。。组缺血区更／全心重和坏死区重／全心重与对照组比较呈明显下降（［丫0．of 或P＜0．05）。（4）红花FQ。各组给药后15tilln均表现出不同程度的增加心脏冠脉流量u＜0．“或P＜O．01，心肌耗氧量明显减少（P＜O．01＜O．OOI。①）红花厂卜各组对心肌缺血大鼠血流动力学有明显改善作用u①．05或R0．of人红花［’。。（20mg、40mg ．kg叫）组心肌中 MDA含量较对照组明显减少（P＜0．01），SOD汛性I‘偕（I，＜0．01），并呈现剂量相关性。结论： 红花F。。能明显减慢心率，改善心脏血流动力学指标，减少心肌缺血的范日，保护缺血心肌；增加冠脉流量，减少心肌耗氧量，并表现出剂量相关性变化，其机制部分可能与抗氧自由基有关。更多还原

【Abstract】 0bject i ve: To observe the effects of Carthams tinctorius F Q 2 (SY F Q 2 ) onthe experimental myocardial ischemia model and investigate its mechanism.Methods: Part one Acute myocardial ischemia (AMI) was induced by acuteobstrUct of left anerior descending coronary artery 30 healthy hybrid dogs wererandomly divided into contro1,Salvia miltiorrhizae (SM0.8g. kg-’ iv), SY FQ 2(5mg. kg-’), SY F Q 2 (l0mg. kg’), SY F Q 2 (40mg. kg’) grouP. The changes ofHR. STffe. ST-T period in ECG wee taken down befOre ligation and post-treatffiellt of SY F Q 2 (5min, l5min, 30min, lh, 2h, 3h, 4h) respectively All piecesof myocardia was dipped into the liqllid of Nitroblue tetrazolium peBT),Totalheart weight (TW), risk zone weigh (RZW), infarct zone weigh (IZW) andIZW/TW were weight respectivelyPart two AMI model were induced by isoproterenol (l0mg. kg-’ ). Thechanges of indexes on hemodynaxnics in rats were observed, such as MBP, LVSP,LVEDP and idPMax. After this experiment, the content of MDA and theactivity of SOD in the plosma and myocardial tissues were measured.ResuIts: (l) The heart rate of SY FQ 2 (5mg. kg-’) grouP was slown downsignificantly (P<0.005),but there were no significant variation in SY F a 2 (20mg, 40mg ?kg"1) group.(2) The elevation of ST-T period induced by AMI was inhabited remarkably in SY F a 2 (5mg, 20mg ?kg"1) and SM(0.8g ?kg"1) groups (PO.05 or P<0.01), and the variation of ST-T period had changed notably in SY F a 2 (5mg *kg"’) and SM(0.8g ’kg’1) group. Compared with control group, RZW/TW and IZW/TW can declined significantly (PO.01 or P<0.05).(3)SY F a 2 (20mg ?kg"1) can improve rats hemodynaics. Compared with control group, the content of MDA and the activity of SOD in the myocordium were decreased in SY F a 2 (20mg,40mg ?kg"’) groups (PO.05 or PO.01).Conclusion: SY F a 2 can decrease HR significantly and improve the index of hemodynamics and decline the risk zone, and their mechanism may be partly related to oxygen free radicals directly or indirectly.更多还原