【作者】 张洪波；

【导师】 金敖兴；

【作者基本信息】 安徽医科大学 ， 影像医学与核医学， 2002， 硕士

【摘要】 目的：研究同步辐射CK、OK软X射线(E_CK=0.28kev、E_OK=0.52kev)对质粒PBK-CMVDNA的辐照生物效应。 方法：以⁶⁰CO-γ射线为对照，把质粒PBK-CMVDNA放置在氮化硅样品室中。在含水的溶液状态，均匀接受CK、OK软X射线辐照，然后将其转入大肠杆菌XL₁-Blue中，分别以突变率与存活率为指标，观察CK、OK软X射线对质粒PBK-CMVDNA的辐射诱导突变作用及其对转化活性的影响。 结果：CK、OK软X射线致质粒PBK-CMVDNA转化活性降低的能力分别是⁶⁰CO-γ射线的3.67和3.35倍，对质粒PBK-CMVDNA的诱变作用分别约是⁶⁰CO-γ射线的2倍。CK在转化活性能力和诱变作用的能力方面稍高于OK软X射线。 结论：与⁶⁰CO-γ射线相比，CK、OK软X射线具有高效生物效应，CK软X射线在生物学效应方面稍高于OK软X射线。更多还原

【Abstract】 Objective: To study the effects of radiation of synchrotron produced 0.28kev carbon k soft X-ray and 0.52kev oxygen k soft X-ray on the plasmid PBK-CMV DNA with 60CO-y ray as reference radiation.Methods: By using a special designed SijN* sample chamber plasmid PBK-CMV DNA was exposured to CK OK soft X-ray in water solution and then transformed into the E. Coli XL 1-Blue. The change of average biology survival rates and mutation frequency were investigated as the endpoints to study the radiation effects of CK^ OK soft X-ray on the plasmid PBK-CMV DNA.Result: The ability of CK^ OK soft X-ray leading to the decrease of average biological survival rates of plasmid PBK-CMV DNA was 3.7 and 3.14 times as that of 60CO-y ray respectively. Mutation frequency of plasmid PBK-CMV DNA caused by CK, OK soft X-ray was twice as that of by 60CO-y ray .At same time ,the data showed CK softX-ray had a better effect than OK soft X-ray Conclusion: The experiment result show the average biological survival rates of CK- OK softX-ray are more three times less than that of 60CO-y ray. The mutation frequence of CK, OK soft X-ray are twice higher than 60CO-y ray. The biological effects of CK soft X-ray is more effective than that of OK soft X-ray.更多还原