【作者】 黎永学；

【导师】 张德纯；

【作者基本信息】 重庆医科大学 ， 临床检验诊断学， 2002， 硕士

【摘要】 利用原生质体融合技术，将自制的双歧杆菌和酿酒酵母的原生质体进行细胞融合，初筛出具有双歧杆菌和酿酒酵母生物学特性、较稳定的融合细胞株BSF1和BSF2，克服了双歧杆菌因厌氧特性而不易开发利用的难题。双歧杆菌和酿酒酵母原生质体融合是厌氧菌和需氧菌的细胞融合，也是原核细胞和真核细胞的跨界融合，在国内外还未见相同文献报道。它的研究成功，为尔后进一步研究双歧杆菌菌种改良极其生物学功能的开发应用指出了新的途径。更多还原

【Abstract】 The present thesis intends to discuss the solving of a difficult problem of developing and utilizing Bifidobacterium. By using the protoplast fusion technique, a pair of fusant BSF1 and BSF2, comparatively stable and having a biological character of both Bifidobacterium and Saccharomyces cerevisiae, are obtained when making a cell fusion of a homemade Bifilobacterium and Saccharomyces Cerevisiae Protoplast. The success of this research overcomes the difficulty in developing and utilizing Bifidobacterium because of its anaerobic character.The fusion of Bifidobacterium and Saccharomyces Cerevisiae Protoplast is not only a cell fusion of anaerobic bacteria and aerobic bacteria, but also an inter-kingdom fusion of prokaryotic<WP=6>cell and eukaryotic cell. As far as the author of this thesis has noted, no similar or related researches and achievements in this field have ever been reported both abroad and at home. Its success undoubtedly offers new ways in the study of bifidobacterium species’improvement as well as the development and utilization of its biological function in the future.更多还原