【作者】 张莉滟；

【导师】 张德纯；

【作者基本信息】 重庆医科大学 ， 临床检验诊断学， 2002， 硕士

【摘要】 双歧杆菌作为人和动物肠道内最重要的优势生理菌群，有着其它生理菌无法比拟的生理活性，可用作日常饮料和食品的添加剂，用于机体胃肠道功能紊乱等疾病的防治，因而有着巨大的潜在应用价值。然而双歧杆菌作为严格的厌氧菌，暴露在空气中易死亡，给其开发和应用带来不便。本实验以兼性厌氧乳杆菌作为遗传物质的供体，应用原生质体融合技术来改良双歧杆菌菌种厌氧特性。将乳杆菌耐氧基因随机整合到双歧杆菌中以改变或提高其耐氧性能，并使融合菌株集双亲优良遗传性状为一体。融合子的成功筛选表明通过双歧杆菌和乳杆菌原生质体融合能够显著改变双歧杆菌厌氧状况，从而更有利于双歧杆菌的开发应用。更多还原

【Abstract】 Bifidobacterium, which constitute one of the predominant bacterial groups of human and animal intestines, have stimulatedthe interest of many investigators not only because of their function in physiological role but also their potential use in people’s daily life. They can be used as dietary adjuncts in dairy foods and in prophylactic and therapeutic preparations for human and animal gastrointestinal disturbances. However, Bifidobacterium, which are exposed to the air, soon die because of their obligate anaerobic character. For this it is inconvenient or disadvantageous to exploitation and applications of Bifidobacterium. Utilizing the technology of protoplast fusion, facultative Lactobacillus’s aerotolerant gene was integrated<WP=6>into Bbifidobacterium’s chromosomes randomly to improve their tolerance of aerobic life or change their obligate anaerobic character. The result shows that this can be completed by the technology of protoplast fusion in Bifidobacterium and Lactobacillus. In addition, fusants are more convinent to exploitation and applications of Bifidobacterium in the near future.更多还原