【作者】 於朝梅；

【导师】 王恬；

【作者基本信息】 南京农业大学 ， 动物营养与饲料科学， 2002， 硕士

【摘要】 为研究乳源性生长因子对动物胃肠道生长发育的作用，本试验以离体犊牛小肠上皮细胞为试验对象，比较研究了酪蛋白和配方乳的酶(胃蛋白酶和胰蛋白酶)解产物对离体犊牛小肠上皮细胞的增殖、葡萄糖4吸收和细胞膜酶活性的影响。试验表明，酪蛋白的酶解产物促细胞增殖的效果不显著，反而降低了细胞的增殖率，但酪蛋白酶解产物对提高细胞吸收葡萄糖的能力有很强的促进作用，对细胞膜碱性磷酸酶活性影响不显著(P＞0.05)，对乳糖酶活性影响在水解60分钟时作用最佳，对Na~+-k~+-ATP酶活性在所有酶解产物中都有提高(P＜0.05)。配方乳的胃蛋白酶和胰蛋白酶酶解产物在胰蛋白酶解至60分钟以后时对细胞增殖率的影响较未消化的配方乳相似，其余酶解产物均提高了细胞的增殖率(P＜0.01)，在促进细胞吸收葡萄糖方面有很显著的作用(P＜0.01)，各试验组皆提高了细胞乳糖酶的活性。本研究证明，酪蛋白和配方乳经适当的酶解处理可将隐含在其中的活性肽逐渐释放出来，酶解的酪蛋白和配方乳具有刺激离体小肠上皮细胞增殖和功能发育的活性。有关酶解酪蛋白和配方乳的生物学效应和分子生物学机制还有待进一步研究。更多还原

【Abstract】 To find out the effects of milk-borne growth factors on gastrointestinal development, studies were conducted to examine the effects of the pepsinized casein(PC), trypsinized casein(TC), pepsinized formula milk(PFM) and trypsinized formula milk(TFM) on the proliferation, the glucose absorption and the enzyme activities of epithelium in small intestine of calves. Results showed that PC and TC reduced the rate of epithelia proliferation, facilitated the absorption of glucose by cells and increased the activity of Na+-K+-ATPase (P<0.05). But they have no impact on the activity of alkaline phosphatase (P>0.05). The activity of lactase was the highest when treated with casein that had been pepsinized or trypsinized for 60 minutes. In addition, both PFM and TFM significantly increased the rate of epithelia proliferation (P<0.01), the absorption of glucose (P<0.01)and the activity of lactase, with the exception of the formula milk that had been trypsinized for more than 60 minutes, which had the same effect on epitheliumproliferation as untreated formula milk. These results suggested that the active peptides can be released from casein and formula milk treated appropriately by enzymes, and enzyme treated casein as well as enzyme-treated formula milk can stimulate epithelium proliferation and the development of epithelium function in vito. The biological effects and the mechanism of molecular biology of the enzyme-treated casein and formula milk should be studied further.更多还原