【作者】 李建文；

【导师】 陈代文； 张克英；

【作者基本信息】 四川农业大学 ， 动物营养与饲料科学， 2002， 硕士

【摘要】 本文研究了在正常和LPS诱导的免疫应激条件下10kg断奶仔猪可消化赖、蛋、色、苏氨酸的平衡模式。选择30头9.03±0.63已阉割的长白(公)×荣昌(母)断奶仔猪，分为正常和应激两个组，每组15头猪。采用Wang和Fuller(1990)的氨基酸部分扣除法，单因子试验设计，进行氮平衡试验和消化试验。氮平衡试验设5个处理，即低蛋白加合成氨基酸正对照组(PC)和在PC基础上逐一扣除25％待考查的氨基酸的四个试验组。将15头猪平均分配到5个处理组中，每个组3头猪(3个重复)。应激组在试验的第0、2、4和6天颈部肌肉注射LPS，而正常组则只注射生理盐水。消化试验从氮平衡试验的第三天开始，给正对照组的6头猪试粮添加三氧化二铬作为指示剂，试验结束后用屠宰法回肠末端取样测定氨基酸的表观消化率。实验结果表明，按每头每天每千克代谢体重的蛋白质沉积量和回肠表观可消化氨基酸摄入量来计算，应激条件下四种氨基酸的平衡比例为DLys 100、DMet 27、DTrp 29、DThr 59；正常条件下为DLys 100、DMet 30、DTrp 21、DThr 61。表明仔猪处于正常和应激条件下的理想氨基酸模式存在差异，应激条件下蛋氨酸和苏氨酸略有下降而色氨酸的比例有较大幅度的提高。更多还原

【Abstract】 Two nitrogen-balance experiments were conducted to determine the optimum balance pattern among the four ammo acids (lysine,methionine,tryptophon and threonine) in the diet on digestible basis for piglets (10kg) challenged with lipopolysaccharide or with saline as control.The method of amino acid deletion was adopted.All pigs were of a single genetic strain and geographical site of origin.In each group fifteen Landrance x Yongchang castrated piglets were allotted randomly to one of five groups of dietary amino acid regimens. Positive control group was supplemented with four amino acids. One of the four amino acids was deleted by about 25% in the respective treatment groups. After the nitrogen-balance experiments,the slaughter technigue was introduced to determine the ideal amino acid digestibility of the positive control by using chromium oxide( 3g/kg) as the indicator. The results showed that the ideal amino acid pattern of Lys/Met/Thr/Trp on the digestible basis was 100/27/29/59 for 10- kg pigs under immunologic stress, and 100/30/21/61 for piglets under normal condition.更多还原