【作者】 余惠旻；

【导师】 刘塔斯； 肖小河；

【作者基本信息】 湖南中医学院 ， 中药学， 2002， 硕士

【摘要】 中药四性是中医药最基本的理论之一，长期以来一直是中医药基础研究的难点和热点。本专题从生物物理化学和分子生物学的角度，考察了中药四性的客观真实性及其在中医药学中的意义，旨在为中医药现代化特别是中医药理论现代化提供新的思路、方法和依据。 利用微量量热法，测定了大肠杆菌在不同条件下的生长热谱曲线，得到了相应的热动力学参数如生长速率常数（K）、抑制率（I）、半抑制率(IC₅₀)、热焓（ΔH），根据生物热动力学参数值，结合本草文献报道，综合分析了人参（Radix ginseng）、西洋参（Radix quinquefoliae）、红参（RadixGinseng Rubra）药性的差异。结果表明：K、ΔH可作为中药四性研究的物理量指标。微量量热法快捷、灵敏、科学、可行，可作为中药四性理论研究的一种十分有用的手段。 采用逆转录PCR（RT-PCR）技术和斑点杂交技术分别测定了正常大鼠脑组织的腺苷酸环化酶AC（Adenylate Cyclase）的mRNA表达及红参、人参、西洋参对其的影响，从分子水平揭示了不同药性的药物所显示的AC表达的差异性。提示：对AC活性产生兴奋和抑制，从而引起环磷酸腺苷（cAMP）含量发生相应的变化，可能是药物显示寒、热药性的药理机制之一。 本专题采用微量量热法和分子生物学技术研究中药四性理论，在国内尚属首次，对开拓中医药理论研究的思路、寻求中医药理论研究的突破，是一个新的有益的尝试。更多还原

【Abstract】 Four Properties of Traditional Chinese Medicine(TCM) is one of the most essential theory of TCM, and it is the hard and hot point of TCM for longterm. The objectivity and authenticity was inspected by Biophysics,Biochemistry and Molecular Biology, To Provide new ways for the modernization of TCM ,specially for the modernization of the theory of TCM.Microcalorimetry was used to determine the bacterial growth thermogenesis curves in different conditions. The parameters such as multiplication rate constants (k), inhibitory ratio (I), half inhibitoryconcentration (IC50) and heat output (AH) were calculated, from these data,combined with the records of Herbal Literatures, the difference of Four Properties of Chinese Traditional Medicine was synthetically evaluated. Theresult indicated: K and AH were physical parameters to describe the difference of Four Properties of TCM .The methods are quick, sensitive, scientific andfeasible, And it will create practical meaning to the demonstration of Four Properties of TCM.RT-PCR and Dot Blotting were used to examine the mRNA expression in controlling group and treating group. To investigate the AC gene expressionin rats brain and the effect of drugs of warm nature (Radix ginseng Rubra),drugs of neutral nature(Radix ginseng) and drugs of cool nature (Radix quinquefoliae).To declare the difference of AC expression with drugs of different properties on molecular level. The results indicated: the excited and inhibitory course of AC activity causing the change of the content of cAMPwas one of possible mechanism for the manifest of cold, hot nature of TCM.The unity between Microcalorimetry, Molecular Biology and the theoryof Four Properties of TCM is a great pioneering work. It is a beneficial try to expand and search the way of the study of TCM.更多还原