【作者】 朱天淳；

【导师】 王策；

【作者基本信息】 苏州大学 ， 光学工程， 2002， 硕士

【摘要】 基因芯片技术是近年来新兴的生物技术，可用于基因表达分析、疾病诊断、新药研发等多个领域，有极大的应用前景。然而现有的商业化检测仪器价格高昂，限制了芯片技术的推广，而许多精确度和灵敏度要求不高的低密度芯片，不必要用很高灵敏度的检测仪器，只需用简易实用的系统来进行检测。 本文提出了一个用全息滤光片代替通常所用的昂贵的窄带干涉滤光片和二色镜的简易的激光共焦扫描系统，对其应用于基因芯片的检测进行了实验研究。 文中首先介绍了基因芯片技术，然后对本所研制的全息滤光片的性能及其在系统中的作用进行了分析，设计并构建了实际的实验系统，对系统进行了静态测试，确定了系统的检测灵敏度，并编制程序，运用一个数控的X-Y移动平台，对芯片的荧光信号进行动态的扫描检测，重建了荧光信号的二维图象，最后，对结果进行了讨论并提出了今后改进的方向。 本文研究的结果表明，将全息滤光片应用于共焦系统对精确度和灵敏度要求不高的低密度芯片的进行检测，是可以实现的。它的进一步完善，有可能开发成功价格低廉、简易实用的基因芯片扫描仪，促进基因芯片技术的普及应用。更多还原

【Abstract】 Genechip technology as a very useful means of biotechnology has been applied to many research areas such as gene discovery, disease diagnosis and pharmacogenomics etc., but commercially available gene-chip detection instruments are too expensive to be accepted by common labs. Some gene-chips such as low-density arrays do not require very high accuracy and sensitivity, which can be detected by simpler confocal systems.In this paper, a laser confocal scanning system with holographic notch filters used for simple gene-chip detection is presented. The volume holographic notch filters were fabricated in our lab to substitute for the expensive narrow-band interference filter and the dichroic filter in the conventional confocal detection system.At first, the genechip technology was introduced.Then, the characteristic of the holographic filters and their functions in the system were analyzed. The confocal system was designed and constructed, and its sensitivity was determined by static measurement.Next, the gengchips were scanned by using a digital controlled X-Y platform and the fluorescence induced by laser was detected, and the computer reconstructed two-dimensional images of fluorescence intensity.Last, the detection results and the methods of improving the performance of the system were discussed.The experimental results indicate that the confocal system can be used for the detection of some low-density genechips that do not require very high accuracy and sensitivity. It’s possible to develop a cheap and practical genechip scanner with further improvement of the system. That will promote the application of genechip technology.更多还原