【作者】 付蕾；

【导师】 何昭阳；

【作者基本信息】 吉林农业大学 ， 预防兽医学， 2002， 硕士

【摘要】 本研究旨在探索鸡新城疫泄殖腔免疫的可能性和可行性，并与滴鼻、饮水等免疫方法比较。采用新城疫Lasota弱毒疫苗于14日龄和28日龄通过泄殖腔、滴鼻、饮水免疫雏鸡，并设对照组。免疫后，取血清、泪液、气管液、胆汁、肠液进行IgA含量或HI滴度枪测。结果如下：鸡新城疫泄殖腔免疫7天后，HI滴度和IgA含量均高于滴鼻组、饮水组，但差异不显著(P>0.05)；随后，免疫水下下降，并低于滴鼻组、饮水组，差异仍不显著(P>0.05)。泄殖腔免疫后，消化道局部早现明显的体液免疫应答，肠液中，IgA含量高于滴鼻组、饮水组，但差异不显著(P>0.05)。由此说明鸡新城疫泄殖腔免疫也是一种可以采用的免疫方法。更多还原

【Abstract】 The purpose of this experiment was to research the possibility and feasibility of immunization to chicken with ND vaccine by the route of the cloacal compared with the route of nasal, oral etc. The chickens were immunized with ND attenccated vaccine by dropping cloacal, nasal, oral at 14 and 28 days old, and the control groups were set up. The IgA content in serum, tear, tracheal fluid, bile and intestinal fluid and HI liter in serum, tear, tracheal were dectected after immunization .The results were as follows. Antibody contents in chickens immunized ND vaccine by the cloacal after 7 days were higher than that by the nasal, oral, but the difference was not significance. Subsequently, the immunoglobulin content descended and was lower than that by the nasal, oral and the difference was not significance either .The local alimentary canals of the immunized chickens were showed humoral immune response obviously by the cloacal, the content of IgA in intestinal fluid was higher than that by the nasal, oral, the difference was not significance .The experiment results indicated that chicken could be immunized by the route of cloacal with ND vaccine.更多还原