微生物生长及生化过程的压电传感技术的研究与应用

【作者】 张书芬；

【导师】 魏万之；

【作者基本信息】 湖南大学 ， 分析化学， 2002， 硕士

【摘要】 压电声波传感技术因其灵敏度高、响应谱广、结构简单和成本低廉等特点已经被广泛应用于分析化学、生物化学、环境监测及分子生物学等众多领域。本文充分利用单面触液型压电传感技术对溶液粘度和密度的响应以及串联式声波传感器(SPQC)对溶液电导率和介电常数的响应，与微生物生长和生化反应特征相结合，对几个新的体系进行了理论与应用方面的研究。在下述四个方面取得了一些创新性的成果： 1．采用声波阻抗分析技术，基于某些抗生素类药物对微生物生长的抑制作用，建立了测定废水污染程度的生物学新分析方法，并用于环境废水污染程度的监测。 2．根据动态电阻与溶液粘度和密度变化的关系，结合Logistic种群生长模型，首次建立了抗生素类药物抑制微生物种群生长的的阻抗响应模型，通过对实验数据的拟合，估计了与微生物生长相关的三个抑制参数。 3．利用阻抗响应曲线提供的信息，以溶菌酶为代表酶，研究了静磁场对酶活性的影响。首次提出了包含磁化时间和磁场强度变量的响应模型。实验结果表明在一定的磁场强度和作用时间内，模型反映了体系的真实行为。 4．利用SPQC传感器对溶液电导率的灵敏响应，探讨了了一定强度的静磁场对大肠杆菌的生长状况的影响。在理论分析的基础上，导出了相关的频移响应模型，估计了有关参数。更多还原

【Abstract】 Piezoelectric acoustic wave sensing technique has been applied to numerous fields, including analytical chemistry, life science, environmental monitoring and surface sciences because of it’s small size, high sensitivity, simplicity, low power consumption and broad sensing spectrum. While integrating with the characteristics of microbial growth and biological reaction, this thesis presents a theoretical and applied study of several new system based on the response of one-face contacting liquid piezoelectric quartz crystal sensor to the viscosity and density of solution, and the response of SPQC sensor to the conductivity of the solutioa The creative works are summarized four aspects as follows:1. New method for determination the environmental waste water was established by bulk acoustic wave impedance analysis technique, which is based on the inhibition of antibiotics to the growth of bacteria. The proposed method was applied to monitor the environmental wastewater.2. An impedance response model about the inhibition of antibiotics to microorganism popular growth was established first time. The model was based on the relationship between the motional resistance and the viscosity and density changes of solution, combined with the Logistic popular growth model. The three inhibition parameters were estimated by fitting the experimental data.3. Using lysozyme as a model, the effect of static magnetic field on me activity of lysozyme was studied using information provided by impedance response, two response models were first time proposed, which contained the parameters of magnetic field intensity and the magnetization time of lysozyme, respectively. The experimental results indicated that the model reflected the real reaction of the detection system in a certain intensity of magnetic field and magnetization time.4. The effect of static magnetic field on the growth of Eschrichia coli was discussed by the sensitive response of SPQC sensor to the conductivity of solutioa A relative frequency shift response model was derived on the basis of theory analysis and the relative parameters were estimated.更多还原