【作者】 黄学洙；

【导师】 严相默；

【作者基本信息】 延边大学 ， 麻醉学， 2002， 硕士

【摘要】 目的：研究不同浓度乙醇及酚甘油行硬膜外阻滞致脊神经及脊神经根破坏超微结构变化；方法：20只家兔随机分为四组，A组为正常对照组，注入生理盐水，B组注入75％乙醇0.5ml，C组注入无水乙醇0.5ml，D组注入10％酚甘油0.5ml，注药速度为0.5ml／min。穿刺注入完毕后，在同样条件下饲养并观察7d。7d后灌流固定，取脊神经和脊神经根应用JEM1200EX透射电镜下观察并摄片。结果：75％乙醇组脊神经线粒体变性、轴突微丝、微管消失，脂褐素颗粒增多，无髓纤维溶解，脊神经根有髓纤维脱髓鞘样改变，Schwann细胞肥大：无水乙醇组有髓纤维轴突内线粒体减少，无髓纤维线粒体空泡样改变，脊神经根变化不明显，10％酚甘油组脊神经和脊神经根变化不明显。结论：硬膜外NNB最佳药物是75％乙醇，硬膜外NNB的效果不明确。更多还原

【Abstract】 Objective:To study the changes of ultrastructure induced by epidural blockade using different concentration of alcohol and phenol glycerin.Methods:20 rabbits were randomly assigned into four groups. Group A was control group,recieved a epidural injection of normal saline.Group B recieved a epidural injection of 75% of alcohol. Group C recieved a epidural injection of dehydrated alcohol and group D 10% of phenol glycerin. The speed of injection was 0.5ml/min.After the puncture and injection,the rabbits were raised under same condition for 7 days. Then a perfused fixation was performed and the spinal nerve and the roots of spinal nerve were taken and observed and shot using a JEM1200EX transmission electro-microscope.Results:Mitochondrials of spinal nerve were denaturalized,microfibril and microtubule of axonemes were vanished,lipofuscin granules were increased,non-myelinated fibrinolysis was occured and myelinated fibre root of spinal nerve were foundeddemyelinated,and hypertrophy of Schwann cells was observed in thegroup of 75% of alcohol. The mitochondrials were decreased in axons ofmyelinated fibre,vacuoles were observed in mitochondrials ofnon-myelinated fibre,and no significant changes was observed in the root of spinal nerve. No significant changes was observed in spinal nerve and root of spinal nerve in 10% of phenol glycerin group. Conclusion:the best dood concentration of drug for NNB is 75% of alcohol,and theefect of epidural NNB is undefined,更多还原