【作者】 马秀玲；

【导师】 陈盛；

【作者基本信息】 福建师范大学 ， 高分子化学与物理， 2002， 硕士

【摘要】 为制备用于固定化酶的磁性壳聚糖微球，本研究采用化学共沉淀法制备磁流体—Fe₃O₄水合物，在磁流体存在下，以反相悬浮聚合法合成磁性壳聚糖微球，并用TEM、SEM、IR、FM—2型永磁天平等对其进行了表征。然后，以磁性壳聚糖微球及壳聚糖微球分别作为载体固定辣根过氧化物酶，结果表明：磁性壳聚糖微球固定的辣根过氧化物酶的理化性质优于壳聚糖微球固定的辣根过氧化物酶，而且磁性酶因具有磁响应性，而可方便、快速地从反应体系中分离。最后，初步探讨了磁性壳聚糖微球固定化辣根过氧化物酶对模拟含酚废水的催化氧化效率，结果表明：磁性壳聚糖微球固定化酶的反应活性比原酶和壳聚糖微球固定化酶的高。本实验对含酚废水的处理进行了有益的尝试，且为辣根过氧化物酶的固定化提供了一种新的载体，该载体在生物化学领域有重要的意义和潜在的应用前景。更多还原

【Abstract】 The purpose of the present study was to prepare Magnetic Chitosan Microspheres that were used as a carrier to immobilize Horseradish Peroxidase(HRP). Firstly, the magnetic particles(Fe3O4 hydrate) were prepared by coprecipitation, then Magnetic Chitosan Microspheres were synthesized by reverse-phase suspension polymerization in the presence of magnetic particles. The carrieres and magnetic particles were characterized by TEM* SEM> IR, FM-2 Magnetic Balance and so on. Secondly, as a carrier, the immobilization of HRP on Magnetic Chitosan Microspheres was studied. In constrast to magnetic immobilized enzyme, the immobilization of HRP on Chitosan Microspheres was also studied. The results showed that Magnetic Chitosan Microspheres immobilizing HRP had the better physicochemical properties than native HRP and Chitosan Microspheres immobilizing HRP, and because of its magnetic responsibility, it was simple and convenient to retrieve magnetic HRP. At last, the catalytic oxidizing efficiency of Magnetic Chitosan Microspheres immobilizing HRP was studied. It was found that the reaction activity of Magnetic Chitosan Microspheres immobilizing HRP was superior to Chitosan Microspheres immobilizing HRP and native HRP. This experiment was a useful attemptment to deal with Phonelic Wastewater, and the new carry showed an important value in biochemical.更多还原