【作者】 李坤武；

【导师】 钟秀会； 张继东；

【作者基本信息】 河北农业大学 ， 临床兽医学， 2002， 硕士

【摘要】 给300日龄、体重2000～2500g的“京白939”产蛋母鸡饲喂高脂肪饲料，复制鸡脂肪肝综合征(Fatty liver syndrome，FLS)病理模型，通过检测血清甘油三酯(TG)、总胆固醇(TC)含量，肝脏脂肪含量、肝重╱体重值，以及血清谷丙转氨酶(GPT)和谷草转氨酶(GOT)活性，并观察光镜下肝组织的病理学改变，研究了FLS患病鸡脂肪代谢的变化和中药制剂三征肝泰对FLS的防治效果。结果显示：①高脂肪饲料可使造模各组血清TG、TC浓度，肝脏脂肪含量、肝重╱体重值，以及血清GPT和GOT活性较健康组和试验前明显升高(P＜0.01或P＜0.05)，光镜下可见肝细胞内有大的脂肪滴存在，细胞肿胀，肝窦消失。试验共使用三种高脂肪饲料，其中高脂肪饲料Ⅰ所造病理模型最为理想。②三征肝泰预防用药对高脂肪饲料导致鸡血清TG、TC的病理性升高具有明显抑制作用，能显著降低肝脏脂肪含量和肝重╱体重值，减轻肝细胞水肿、坏死的病变程度，降低血清GPT、GOT活性。其中预防D组效果最佳，试验期间产蛋率维持在71.78％左右。试验结束肝脏脂肪含量仅占整个肝重的27.74％，血清TG、TC浓度(mg╱dl)为662.16±177.98和137.50+25.69，血清GPT、GOT活力(U╱L)为16.69±0.45和210.5±43.54，各项指标均明显低于模型B组同期水平(P＜0.01)，与健康A组比较，仅血清TC存在显著差异(P＜0.05)，其它各项指标差异均不显著(P＞0.05)。与阳性对照F组相比，差异极显著(P＜0.01)。③三征肝泰治疗用药可显著降低肝脏脂肪含量和血清中TG、TC含量，减轻肝细胞水肿、脂肪变性程度，降低血清GFT、GOT活性。其中治疗D组效果最佳，试验结束，产蛋率恢复至50％，肝脏脂肪含量下降至26.7％，血清TG、TC浓度比给药前下降63.1％和67.6％，血清GPT、COT活力比给药前下降19.7％和23.2％，各项指标均明显低于模型组同期水平(P＜0.01)，与健康组比较，仅血清TC存在显著差异(P＜0.05)，其它各项指标差异均不显著(P＞0.05)。与阳性对照F组相比，差异不显著(P＞0.05)。上述试验结果表明：①利用高脂饲料可成功复制鸡FLS病理模型。②中药制剂“三征肝泰”可明显提高鸡的产蛋率。③中药制剂“三征肝泰”对由高脂肪饲料引发的血脂升高和肝脏脂肪变性具有明显的预防和治疗效果。④中药制剂“三征肝泰”对肝细胞损伤具有明显的保护作用。更多还原

【Abstract】 Fatty liver syndrome (FLS) models were prepared by feeding the hens ,which were 300-day-old and averaging 2000-2500 g of body weight,on high-fat diet. We studied the curative and the preventative effects of a Chinese herbal medicine prescription "San Zheng Gan Tai" on FLS and the changes in the fat metabolism of the sick hens through testing the TG, TC and the GPT, GOT levels in the serum and the liver fat content, the liver/body weight ratio and also through testing the pathological changes of the liver. The results showed that: 1. The levels of TG, TC and GPT, GOT in the serum and the liver fat content, the liver/body weight ratio of the model group were significantly higher when compared with the control group (PO.01 or PO.05). Fat drop was seen in the liver cells which were swelling observed under the light microscope. Three high-fat diets were used and the model made by high-fat diet is more ideal. 2. The preventative group was fed on high-fat diet with an addition of "San Zheng Gan Tai", "San Zheng Gan Tai" can significantly lower the liver fat content and the TG, TC in the serum and also can remarkably lessen the steatosis degree. Group D is the best during the experiment, laying-egg ratio is 71.78% and at the end of the experiment the liver fat content is 27.74%, TG, TC ( mg/dl ) is 662.16+177.98, 137.50+25.69, GPT, GOT ( U/L ) is 16.69+0.45, 210.5+43.54,respectively.AH these are lower than that of model B at the same period (PO.01), compared with healthy group A, only TC has significant difference (PO.05), others’ differences are not significant (P>0.05), and compared with positive control group F, differences are very significant (PO.01). 3. Cure with "San Zheng Gan Tai" not only significantly lowered the liver fat content and the TC, TG, GPT, GOT of serum but also remarkably lessened the stealosis degree. The curative effect of group D is the best, at the end of the experiment the laying-egg ratio is recovered to 50%, the liver fat content is lowered to 26.7%, TC, TG, GPT,GOT of serum is lower than that of the model group at the same period (PO.01). Compared with healthy group only TC has significant difference (PO.05), none of the other indexes were significantly different (PX).05).All these results implied that (1) FLS models can be made successfully by feeding the hens on high-fat diet; (2) Chinese herbal medicine "San Zheng Gan Tai" can raise the laying-egg ratio; (3) "San Zheng Gan Tai" has significant preventative and curative effects on FLS, and (4) "San Zheng Gan Tai" can prevent the liver cell injury.更多还原