【作者】 张胜利；

【导师】 安守芹；

【作者基本信息】 内蒙古农业大学 ， 水土保持与荒漠化防治， 2002， 硕士

【摘要】 桃(prunus persica(L.)Batsch.),蔷薇科李属植物。由于优质桃的上市相对集中，鲜桃又不耐贮存，罐藏成为桃的重要加工贮藏方式。粘核种肉质较致密，果肉成熟度和果肉硬度内外较均匀一致，粗纤维少，罐藏时挖核后核洼光洁，不易产生沉淀物，所以培育粘核桃品种具有重要的经济价值。桃的质量性状离核和粘核分别由基因F和f控制，F对f完全显性，本研究以桃品种京玉、美味及其杂交后代为材料，通过对F基因RAPD标记的SCAR转化，为桃育种工作的早期选择和重要经济性状的有效利用打下基础。本试验采用CTAB方法提取桃基因组DNA，优化了RAPD反应体系，从I-07引物的5′端反向延长一个碱基合成一引物，对京玉、美味及其后代进行检测，表现出多态性。樱桃(Cerasus sp.)，蔷薇科樱桃属植物。成熟早，果艳，味美，深受消费者欢迎。由于寿命长，且主要采用嫁接繁殖，不同地域间经常交换品种，导致同名异物和同物异名现象严重。因此对品种进行可靠的鉴定，对于推广优良品种、保护育种工作者的权益具有重要意义。本实验采用RAPD技术，用筛选的7个多态性引物，区分了樱桃的18个品种，并进行了相似性系数的分析，初步研究了品种之间的亲缘关系，为育种亲本的选择提供了理论依据。更多还原

【Abstract】 Peach (Prunus persica (L.) Batch.) originates from China. Mature period of quality peach appears in a short time. Storage property of fresh peach is poor. Canning becomes an important way in peach processing and storage. Breeding peach with this qualitative trait whose pit can separate from flesh easily have economic significance for the same maturity and stiffness inside and outside of flesh; the compact flesh of fruit; low coarse fiber content and few sediments in can. This qualitative trait and its corresponding trait are controlled by F and f gene. F gene is dominant. Transforming RAPD marker linked to the F gene into SCAR marker was studied using "JINGYU" "MEIWEI" and their hybrid progeny. This work laid a foundation for early selecting parents and makes full use of some important economic trait of fruit trees. Genome DNA was extracted with CTAB method. RAPD reaction system was optimized. A primer which add a base from 5′end of OPI-07 was synthesized. It was used to test "JINGYU" "MEIWEI" and their hybrid progeny. Polymorphism was appeared as length polymorphisms that convert RAPD loci into co dominant. Cherry (Cerasus sp.), its fruit is welcomed for delicious taste, early mature period and beautiful color. Mainly grafting in its long life propagates it. Its cultivars are often exchanged in different regions. As a result, different cultivars have the same name and the same cultivars have many different names. So it must be identify varieties accurately. This work is especially important for popularizing quality cultivars and protecting breeders′interest. In this article, 7 primes were selected to differentiate 18 cherry cultivars by RAPD. Similarity Coefficient was analyses. The genetic relationship among cultivars was studied preliminarily. This study provided scientific basis for selecting parent.更多还原