复杂荧光多组分体系中二阶标准加入及二阶校正化学计量学算法的基础研究

【作者】 龙宁；

【导师】 吴海龙；

【作者基本信息】 湖南大学 ， 分析化学， 2001， 硕士

【摘要】 本论文在绪论部分阐述了化学计量学的产生背景、发展历史，并展望了其发展前景；同时在文献综述的基础上，选取二阶标准加入法及其在实际分析体系中的应用和二阶校正方法在荧光体系中的应用作为自己的研究方向，通过模拟计算讨论了二阶标准加入法应用过程中各种因素对分析结果准确度的影响，并将之应用于实际体系的测定，取得了较好的效果。 第二章用模拟讨论了二阶标准加入法中算法对分析结果的影响，挑选了几个有代表性的算法，从分析体系特点（光谱本身的共线性）、噪声水平、组分数的选择几个方面进行了分析讨论，发现各种算法各具特色，只有根据实际情况选择恰当的算法用于二阶标准加入法的立体阵的解析，才能获得最佳结果。 论文第三章，主要讨论了标准加入的次数、加入的量的多少、移液过程引入的误差及量测过程引入的误差这些重要因素对分析结果准确度的影响，获得一定的观察结果，可供实验设计时作为参考。 第四章和第五章是两个二阶标准加入法的实际应用，第四章用SOSAM法对复合维生素B片中的VB₂及VB₂和VB₆进行了测定，结果令人满意；第五章用SOSAM法对干扰共存下的两种多环芳烃进行了同时测定。 在第六章中，采用二阶校正的方法对对干扰物对氨基苯甲酸（PABA）存在下的水杨酸（SA）和2，5二羟基苯甲酸（GA）进行了同时荧光测定。还对干扰物水杨酸（SA）存在下的萘普生（NAP）和阿司匹林（ASA）进行了同时测定，结果良好。更多还原

【Abstract】 AbstractIn the introductory part of this thesis, the history and the basic trends of chemometrics have been covered. Based on the review of large numbers of literature, the research direction of the thesis is focused on two parts. One is the error analysis of second-order standard addition and its application, the other is the application of second-order calibration methods in fluorescent mixtures. The influence on the accuracy of analytical results caused by various elements in second-order standard addition was discussed by Monte Carrol simulation. Part of achievement was applied in real analytical determination.In chapter 2, the bias of results brought by algorithms was simulated. Exploiting several typical algorithms, simulation was carried out in such aspects as collinearity of spectra, degree of noise and the number of principal components. The results show that only an appropriate algorithm is chosen in three-way analysis according to the real requirements, could the best results be obtained.In chapter 3, effects of four factors such as the number of standard addition, quantity of standard addition, errors of volume and measurement on the accuracy of results in second-order standard addition method were principally discussed. Some results were obtained which could be regarded as reference in experimental design.The chapters 4 and 5 contain two applications using second-order standard addition. Direct determination of VB2, VB: and VB6 in compound vitamin B tablet by spectrofluorimetry SO^AM was performed with good results. Determination of polycyclic aromatic hydrocarbons, anthrancene and 9,10-dimethylanthrace. in the presence of an unknown interference. 1.2,5.6-dibenzathracene with satisfactory recoveries was carriedout.In chapter 6, two complicated systems were studied using second-order calibrationmethod. Simultaneous determination of salicylic acid and gentisic acid in the presence of /j-aminobenzoic acid by using both excitation-emission fluorescence and second-order calibration based on alternating trilinear decomposition method. Acety (salicylic acid and naproxen in the presence of salicylic acid were also determined.更多还原