【作者】 陈燕；

【导师】 郭云良；

【作者基本信息】 青岛大学 ， 神经病学， 2001， 硕士

【摘要】 目的：检测大鼠局灶性脑缺血再灌注后神经细胞bcl-2、bax和p53 mRNA的表达变化。方法：利用大鼠大脑中动脉缺血模型，采用原位杂交技术分别观察再灌注后2h、6h、12h、24h、2d、3d、7d和14d不同时间点神经细胞bcl-2、bax和p53 mRNA表达状况。结果：缺血再灌注2h在缺血周围区bcl-2mRNA开始表达，24h达高峰，14达假手术组水平；bax mRNA与p53mRNA均于再灌注6h出现，bax mRNA于24h达高峰，3d达假手术组水平，p53 mRNA于24-48h达高峰，7d达假平组水平。高倍镜下可见棕黄色阳性信号位于细胞浆中。细胞核处淡染。结论：大鼠局灶性脑缺血再灌注后bcl-2、bax和p53的表达与缺血性损伤有一定关系，可能参与了神经细胞凋亡的调控。更多还原

【Abstract】 Objective: To study the expression and variation of bcl-2, bax and p53 mRNA in neurons after focal cerebral ischemia / reperfusion in rats. Methods: Using rats model with middle cerebral artery occluded and adopting the technique of in situ hybridization, we detected the expression of bcl-2, bax and p53 mRNA on different time spots(2,6,12,24 hours and 2,3,7,14 days ) after reperfusion of focal cerebral ischemia in rats. Results: In the peripheral area of the infarction, bcl-2 mRNA positive neurons were observed 2h after reperfusion, peaked at 24h, 14d decreased to normal level; bax and p53 mRNA were detected at 6h, they reached their peak at 24h and 48h respectively. In positive cells the plasma is brown not the nucleus. Conclusion: The expression of bcl-2, bax and p53 mRNA in neurons cerebral ischemia / reperfusion was related to ischemic insults, and may play a regular role in neuronal apoptosis.更多还原