电针对大鼠脊髓和内分泌腺内γ-干扰素样免疫阳性物质分布的影响

【作者】 胡格吉勒图；

【导师】 欧阳五庆； 李育良；

【作者基本信息】 西北农林科技大学 ， 基础兽医学， 2001， 硕士

【摘要】 摘 要研究了IFN-γ在大鼠脊髓和内分泌腺中的表达，进而探讨了针灸机理与免疫神经内分泌网络之间的关系。本实验用SD大鼠，随机分为正常对照，电针，免疫抑制，免疫抑制+电针等4组，用G6805-2A探穴针灸仪，电针刺激（刺激强度为3V，频率为2Hz，时间为30min）大鼠后海穴，应用免疫组织化学SP法，观察了在脊髓、垂体、卵巢、睾丸和胰腺内的IFN-γ样免疫反应阳性物质分布的变化，结果如下：1．脊髓中IFN-γ的变化 在正常对照组大鼠脊髓各板层广泛分布有IFN-γ样免疫反应阳性神经元，其中背角胶状质、中间带外侧核和腹核分布较为集中。电针刺激正常大鼠后海穴，脊髓内IFN-γ样免疫反应阳性神经元数量无显著变化。免疫抑制组大鼠脊髓内IFN-γ样免疫反应阳性神经元在总体上呈现下降趋势，尤其是背角胶状质内免疫反应阳性神经元数量明显低于正常对照组。电针刺激免疫抑制大鼠后海穴，脊髓各板层IFN-γ样免疫反应阳性神经元数量，与正常对照组相比无显著差异。上述结果提示SD大鼠脊髓内存在IFN-γ，IFN-γ在脊髓可能起着免疫调节或神经肽样物质的作用。2．垂体中IFN-γ的变化 在正常对照组大鼠垂体前叶广泛存在IFN-γ样免疫反应阳性纤维，而阳性细胞数量极少。电针刺激正常大鼠后海穴对垂体前叶内IFN-γ样免疫反应阳性物质的影响不显著。免疫抑制组大鼠垂体前叶未发现IFN-γ样免疫反应阳性细胞，而阳性纤维密度与正常对照组相比无显著差异。电针刺激免疫抑制大鼠后海穴后阳性细胞数量，与正常对照组相比明显增多，阳性纤维密度显著降低。这说明SD大鼠垂体前叶细胞可能合成IFN-γ，IFN-γ可能是垂体内重要的信息传递物。3．卵巢中IFN-γ的变化 在正常对照组大鼠卵巢内IFN-γ样免疫反应阳性物质分布在卵泡颗粒细胞和黄体内。电针刺激正常大鼠后海穴对卵巢内IFN-γ样免疫反应阳性物质无显著影响。免疫抑制组大鼠黄体内IFN-γ样免疫反应阳性纤维密度明显低于正常对照组，而卵泡内IFN-γ样免疫反应阳性细胞数量略有减少，但无统计学意义。电针刺激免疫抑制大鼠后海穴，卵巢内IFN-γ样免疫反应阳性细胞数量和阳性纤维密度略<WP=4>有上升，但与正常对照组相比无统计学意义。这表明SD大鼠卵巢内存在IFN-γ，可能与卵泡的发育成熟、排卵有密切关系。4．睾丸中IFN-γ的变化 在正常对照组大鼠睾丸间质细胞内少量表达IFN-γ样免疫反应阳性物质。电针刺激正常大鼠后海穴，睾丸内IFN-γ样免疫反应阳性细胞无显著影响。免疫抑制组大鼠睾丸内IFN-γ样免疫反应阳性细胞呈弱阳性，细胞轮廓依稀可见。电针刺激免疫抑制大鼠后海穴，睾丸内出现较多的IFN-γ样免疫反应阳性细胞，但正常对照组相比无明显变化。提示SD大鼠睾丸间质细胞内存在IFN-γ，IFN-γ可能对精子的发育成熟有调节作用。5．胰腺中IFN-γ的变化 在正常对照组大鼠胰岛细胞内存在IFN-γ样免疫反应阳性物质。电针刺激正常大鼠后海穴后IFN-γ样免疫反应阳性细胞数量无显著变化。免疫抑制组大鼠胰岛内极微量表达IFN-γ样免疫反应阳性物质，细胞轮廓模糊不清，无法统计。电针刺激免疫抑制大鼠后海穴，胰岛内出现IFN-γ样免疫反应阳性细胞，但与正常对照组相比无显著差异。提示SD大鼠胰岛细胞内存在IFN-γ，IFN-γ可能调节胰岛内其它激素的分泌。电针刺激免疫抑制SD大鼠后海穴可诱生脊髓、垂体、卵巢、睾丸和胰腺内IFN-γ，这对揭示针灸机理与免疫神经内分泌网络的关系具有重要意义。更多还原

【Abstract】 AbstractThe expression of IFN-γ in spinal cord and endocrine gland in rat was studied and the correlation between acupunture and immuno-neuro endocrine net was discussed . The SD rat were grouped to control group, Houhai point group, immunosuppressive group and immunosuppressive+ electroalupunture (EA) group. The Houhai point of rat was electroacupuntured by using acupunture instrument (G6805-2A, intensity was 3V, frequent was 2Hz, time was 30 miniture), and the changes of the distribution of IFN-γ-like immunoreactive(IFN-γ-li) products in spindl cord, pituitary , ovary, testis and pancreas gland were studied by using the technique of immunohistochemistry SP. The results as follows:1. The change of IFN-γin spinal cord The IFN-γ-li neurons extensively distributed in respective layer of spinal and in control group and the distribution was intensive in the Rolando of dorsal horn, intermediolateral nucleus and ventral nucleus. The number of IFN-γ-li neurons didn’t change obviously after electroacupunturing the Houhai point. The IFN-γ-li neurons showed the descending tendency in immunosuppressive group, especially in the Rolando of dorsal horn. But the number of the IFN-γ-li neurons weren’t obvious different between immunosuppressive+EA group and control group. The results showed that IFN-γexisted in spinal cord of SD rat, and IFN-γ may play the role of immunomodulation or neuropeptide in soindl cord.2. The change of IFN-γ in pituitary The IFN-γ-li fibers extensively distributed in anterior pituitary of control group, and few positive neurons was detected. The effect of EA on IFN-γ-li positive products in pituitary wasn’t obvious in Houhai group.The IFN-γ-li positive cells weren’t detected in anterior pituitary of immunosuppressive group,but the density of positive fibers was similar to the control group. The number of positive cells in immunosuppressive+EA group was higher but the density of positive fibers was lower than control group.The results suggested that the IFN-γ may be synthesized in anterior pituitary in SD rat and IFN-γ may transmit the signal in pituitary.<WP=6>3. The change of IFN-γ in ovary The IFN-γ-li positive products distributed in the granular cell of follicle and corpus luteum of ovary of control group. The change of IFN-γ-like positive products wasn’t obvious in Houhai group.The density of IFN-γ-li positive fibers in ovary of immunosuppressive group was much lower than control group,but the change of positive cells wasn’t obvious in follicle. The density of positive cells and fibers in ovary of immunosuppressive+EA group ascended a little,but it wasn’t obvious compared with control group.The results suggested that the IFN-γin ovary may have closely relationship to the developing mature of follicle and ovulation.4. The change of IFN-γin testis A few of IFN-γ-li positive products were detected in interstital cell of control group. The effect of EA on IFN-γ-li positive cells in testis of Houhai group wasn’t obvious.The IFN-γ-li positive cells in testis of immunosuppressive group was weak stained and the outline of positive cells wasn’t distinct. Many IFN-γ-li positive cells were detected in testis of immunosuppressive+EA group , but which didn’t obviously changed to the control group. The result showedthat the interstitial cell could synthesis the IFN-γ and IFN-γmay modulate the deueloping mature of sperm.5. The change of IFN-γin pancreas gland The IFN-γ-li positive products existed in pancreas islet of control group. There wasn’t obvious changes of IFN-γ-li positive cells in Houhai group. Few of IFN-γ-li positive producbs was detected in immunosuppressive group, and the outline of positive cells wasn’t distinct.The IFN-γ-li positive cells wake presented in pancreas islet of immunosuppressive+EA group, but there wasn’t obvious different between immunosuppressive+EA group. The result suggested that IFN-γ existed in the pancreas islet of SD rat and IFN-γ may modulate the secretion of other hormones in pancreas islet.Electroacupuntu更多还原