溴氨酸生物降解动力学模型的建立

【作者】 刘颖；

【导师】 周集体；

【作者基本信息】 大连理工大学 ， 环境科学与控制工程， 2001， 硕士

【摘要】 本文利用本实验室筛选出的溴胺酸高效降解菌JR-22研究菌的生长过程及溴胺酸的降解过程，绘制了菌生长曲线及溴胺酸降解曲线，根据底物浓度影响试验发现在高浓度时菌JR-22降解溴胺酸过程中存在抑制现象。因此，本文建立了溴胺酸降解过程中细菌JR-22生长及底物消耗的抑制模型，它们分别符合修正的Monod模型及相应的底物降解模型：1)菌的生长模型为，其模型参数μ_max=0.244h^-1,K_m1=791.8mg/l,Ki=231.8mg/l。2)溴胺酸降解模型为，其模型参数分别为：q_max=0.795h^-1,K（m2）=371.7mg/l，K_is=589.1mg/l。根据上述模型提出了一种可能的底物抑制途径；此外，从模型中可以看出，溴胺酸浓度为420～470mg/l时，q与μ均达较高值，这为实际处理工艺提供最佳操作条件。最后，建立了q与μ的关系，并确定了模型参数Y_G=0.194mg菌体/mg溴胺酸，m=0.044h^-1，相关系数r=0.924＞r_0.01（9）=0.735，模型显著。更多还原

【Abstract】 The growth process of Pseudomonas J7R-22, a strain of which can effectively degrade bromanine acid(ABAS), optimum of its cultural condition and degradation process of bromanine acid are studied in this paper. First, the growth curve of the strain and degradation curve of ABAS are drawn from the experiments. It is found that the growth curve of the strain accords with typical growth curve of bacterium and that in rapid growth period JR-22 can degrade ABAS more quickly. Secondly, substrate inhibition exists when JR-22 consumes ABAS. Kinetic inhibition model for JR-22 growth and substrate biodegradation are constructed respectively by renewing Monod Equation and substrate digestion model as follows: 1) ?p ~4S] 1(1 + [S] I K,) (kinetic inhibition model for JR-22 growth), where Jimax 0.244h? Km179 1.8mg/i and Ki231 .8mg/l respectively and 2 ) q = q inax[S] 1(1 + [S] / K1.~) (kinetic inhibition Kn12/(1 + [S]/K,~) + [S] model for substrate biodegradation) where qmaxO.795W? K11~237l .7mg/i, K~~=589.lmg/l. Experimental data are fitted well by these two models and the models can be used in the design of wastewater treatment process. Finally, a reasonable pathway for describing ABAS inhibition is suggested according to the models.更多还原