橙皮甙的提取及其抗氧化作用研究

【作者】 黄燕玲；

【导师】 罗宗铭；

【作者基本信息】 广东工业大学 ， 应用化学， 2001， 硕士

【摘要】 黄酮类化合物具有抗氧化、抗自由基和防癌抗癌等多种生理活性，已越来越受到重视。本文以橙皮为原料，较系统地研究了其中的黄酮物质（主要为橙皮甙）的提取方法，还研究了橙皮甙的抗氧化和抗自由基作用。 主要研究内容及实验结果如下： 1、比较了原料经不同预处理后的提取橙皮甙的效果，发现脱脂处理总提取率降低，但总黄酮得率提高； 2、用6种溶剂对橙皮进行提取试验，结果表明，溶剂亲水性越强，黄酮提取率越高，总黄酮得率也越高； 3、对不同预处理的提取物和不同溶剂的提取物进行抗氧化试验表明，橙皮脱脂后其提取物抗氧化性能提高；水或碱水提取物的抗氧化性能较差，甲醇提取物抗氧化性能最好，乙醇次之； 4、系统地研究了橙皮中橙皮甙的乙醇提取工艺，得到最佳工艺组合为：以80％乙醇室温下提取24小时，乙醇与原料重量比为1：50，并做放大50倍试验验证最佳工艺，得总黄酮得率为2.87％； 5、研究了橙皮甙与增效剂的协同抗氧化效应，发现橙皮甙与抗坏血酸的协同效果最好；用荧光分光光度法研究了橙皮甙的抗羟自由基作用，并与抗坏血酸比较，实验表明橙皮甙可清除羟自由基，在一定浓度范围内清除率随橙皮甙量增大而增强； 6、对橙皮甙化合物的抗氧化机理进行了探讨。更多还原

【Abstract】 Flavones are thought to be more and more important because of their antioxidalive.. anti-free-radical and cancer-pieYenL cancer-resistant activities. In this paper, method of extracting flavones from orange peel is studied systematically, as well as their antioxidative and anti-free-radical activities The main contents and results are displayed as follow1.Extracted hesperidin from orange peel afier different prehandling, found that more flavones were got after dewaxing.2.Extracting hespendin from orange peel by 6 different solvents, results showed that the more hydrophilic the solvent was, the more flavones were got3.Tested the antioxidative effect of all above extracts, found that the extract had better antioxdalive activity if orange peel was dewaxed. The best antioxidative effect was found in extract of methanol followed by alcohol, and extracts of water or basic water had worst antioxdative activity among all the extracts.4.Studied systematically the method of extracting hespeuidin from orange peel by alcohol. The best method was found. using 800/c alcohol as solvent~, 50 times weight as orange peel, extracting for 24 hours at normal temperature.5.Tested aniioxidalive efl~cts when hesperidin from orange peel and dilThrent synergists worked together The results showed that besperidin worked best together with assorbic acid. Tested anti-free-radical effect of hespexidin using fluorescent and compared the effect with that of assorbic acid Found that besperidin can remove free radical, and in certain concentration the larger amount of hespendiri there was, the larger amount of free radical was removed.6. Inquired into antioxidation mechanism of besperidin更多还原