【作者】 曹金山；

【导师】 曹贵方； 李培锋；

【作者基本信息】 内蒙古农业大学 ， 基础兽医学， 2001， 硕士

【摘要】 胆酸（cholic acid，CA）为多种动物胆汁有效成分之一，分子式为 C₂₄H₄₀O₅，分子量为 408.6的无水结晶，mp198℃，[α]_D²⁰+27⁰ pk＝6．4。具有解热、抗炎、抗过敏、抗菌、抗病毒等作用，临床多用于治疗热病神昏、中风痰迷、咽喉肿痛、口舌生疮等症。本实验，采用现代药理学实验方法对胆酸进行了抗炎、镇咳、祛痰、平喘等药理学实验，并测定了炎性介质中PGE₂含量和组织细胞内第二信使cAMP的含量，进一步探讨了CA 的抗炎、镇咳、平喘、祛痰的作用机理。此外还进行了肺组织炎症的治疗和保护等实验。 抗炎实验结果表明，CA按 0．25g/kg和 0．125g/kg剂量，经口服给药后能极显著的抑制耳壳肿胀法、色素滤出法、棉球肉芽肿法、足困肿胀法等方法引起的小鼠及大鼠的炎症和炎性组织中PG₂巳含量。 镇咳、化痰、平喘实验结果表明，CA 按 0.25g/kg和 0．125g/kg剂量，经口服给药后CA 能极显著的抑制用氨水刺激法和二氧化硫刺激法引起的小鼠咳嗽，明显的增加由气管段酚红法测定的小鼠气管内分泌物。2．5％ CA 0．4ml 加入 35ml 麦氏浴槽后能极显著的舒张用气管容积法和气管螺旋条法进行的实验中由磷酸组织胺（His）和5- 羟色胺（5－HT）引起的豚鼠离体气管平滑肌的痉挛。CA按 0．25g/kg和0．125g/kg剂量，经口服给药后能极显著的提高大白鼠气管组织中cAMP水平。 对肺组织炎症的治疗和保护作用实验结果显示，CA 按0．25g/kg和 0．125g八/kg剂量，经口服给药后对二氧化硫刺激法引起的支气管和肺组织炎症有一定的治疗和保护作用。更多还原

【Abstract】 Cholic acid (CA) is one of the effective components of many kinds of animals?bile. Its molecular formula is C24H4005 and the molecular weight of its anhydrous crystallization is 408.6, mp 198 C,[a]D~ +27 0PK~4 CA has the functions of anti- pyretion ~antinflammation~ antialergy~, antibacteria and antivirus and is often used to apoplexy larygopharyngitis and stomatoglossitos Modem pharmacological methods were used in the pharmacological experiments of the antinflammation~ antitusis.. expectoration and antiasthma effects of CA , the content POE2 inflammatory medium and the content of second messenger-cAMP in tissue cell were tested and the mechanism of these actions were discussed .The experiments of treating the inflammation of lung tissue and of protecting its from inflammation were also done. The results of an-inflammatory experiments indicated that when the dosages were 0.25g/kg and 0. 125g/kg and given by oral administration , CA could obviously restrain the inflammation of big mice and little mice and the content of PGE2 which were induced by the methods of Auricular concha swelling ~. Filtering out pigment ~ Cotton ball granular swelling and feet swelling. The results of antitusis~ expctoration and antiasthma showed that with the same dosages and the same way of anti-inflammatory experiments, CA could significantly control the cough of mice caused by NH3 . H20 and SO2 and increase the intratracheal secretion measured by tracheal phenol red test. 2.5% 0.4ml CA was added into Mai Bath-nest and CA could significantly diastole the spasm of guinea-pigs tracheal smooth muscle caused by His and 5-HI with the methods of the Tracheal volume and Tracheal spiral. The level of cAMP was improved when CA was given by oral administration at the dosages of 0.25g/kg and 0.125g/kg. The results of the experiments of the treatment of the inflammation of lung tissue and the protection lung tissue from inflammation showed that when the dosages were 0.25g/kg and 0. 125g/kg and given by oral administration, CA had the functions of treating the inflammation of bronchus and lung tissue and protection them from inflammation.更多还原