电针介导类风湿关节炎免疫耐受的实验研究

【作者】 唐勇；

【导师】 余曙光； 胡玲香；

【作者基本信息】 成都中医药大学 ， 针灸推拿， 2001， 硕士

【摘要】 目的探讨电针对类风湿性关节炎（RA）T细胞耐受的介导作用及其作用机制。方法 采用佐剂性关节炎（AA）作为RA动物模型，电针“足三里”治疗后，测定外周血T淋巴细胞凋亡率、IL-2、CS和胸腺FAS／FAS-L、GCR以及局部炎性物质5-HT、HA和局部痛阈、肿胀度的变。结果 电针组局部痛阈、足跖肿胀程度与模型组相比，呈显著差异；电针组局部5-HT、HA含量明显低于模型组（P＜0．01）；模型组外周血T淋巴细胞凋亡率为29．52％，电针组为43．88％； 模型组血浆C S、胸腺GCR含量下降，电针后显著升高；电针组IL-2含量与模型组比较，有显著性差异（P＜0．01）；电针组胸腺FAS／FAS-L表达亦明显增强，与模型组比较 P＜0．05。结论 电针可提高痛阈、减缓足肿胀；可降低局部炎症组织5-HT、HA含量，抑制炎症反应；电针能够增加外周血T淋巴细胞的凋亡率，介导T细胞耐受；电针可能通过提高外周血C S、胸腺GCR含量水平及IL-2含量，增强胸腺FAS／FAS-L的表达，在神经-内分泌-免疫网络的调节下，介导T细胞耐受。 of 41更多还原

【Abstract】 Objective TO Study T cell tolerance and its mecha-nism on rheumatoidarthritis(RA) mediated with electroacuPuncture(EA). Methods We measuredcontens of 5-HT. HA in affected area and CS. IL-2 \ T lymphocytes apoptosis inblood and Fas/FasL in thyInocyte as welI as GCR by means of ElISA,radioimmunoassay immu-nohistochemstry on adjuvant arthIitis(AA) ammal mode1treated with EA on "Zuanli". Results We fOund that there is a significiantdifference on pain threshold and swelling changes at locality betWeen EA and modelgroup; the contens of 5-HT and HA on affected area in EA group are aPparent lessthap model one(P<0.0l). The aPopotosis rate in AA grouP is 29.52% while in EAone 43.88%; In addition, both the level of CS in blood and GCR in thymocyte in EAgroup are more than that model one, the level of IL-2 in blood shows significantdifference between AA and EA grouP(P<0.05), Moreover, the expression ofFasNasL in thymocyte in EA grouP is more aPparent than in model one(P<0.05),ConcIusion These results suggest that EA can relive swel1ing and stop pain bydiscrease contents of 5-HT and HA in affected area ) on the other hand, EA canincrease T lymphoc}rtes apoptosis rate and mediate T cell tolerance which is possiblemodulated with neuroirnmunoregulation netWork by increasing the qua1ity of CS.GCR and IL-2 and enhance the expression of FasNasL in thymocyte.更多还原