【作者】 张永丽；

【导师】 唐纪良； 李有志；

【作者基本信息】 广西大学 ， 徽生物， 2001， 硕士

【摘要】 水稻白叶枯病菌（Xanthomonas oryzas pv．oryzaee；Xoo）的rpfC缺失 突变体SL3751是一株能诱导水稻对Xoo野生菌株13751引起的白叶枯病 产生抗性的具有潜在防治作用的生防菌株。本研究用cDNA微阵列技术， 经Northern杂交证实，筛选到在SL3751和13751平行接种诱导下感病水 稻金刚30分蘖前苗期的10个差异表达基因的cDNA克隆，对其中4个差 异表达克隆中的外源cDNA片段测序分析发现，这4个克隆中含有4个基 因：细胞色素b5基因、水孔素基因、25SrRNA和与拟南芥一个推断蛋白 基因同源基因。除25SrRNA基因的cDNA，其余3个差异表达基因的cDNA 均为全长。与13751和对照接种诱导处理相比，在SL3571接种诱导的水 稻体内，细胞色素b5基因表达水平低于13751诱导处理，但高于对照处 理下的表达水平。水孔素基因及与拟南芥推断蛋白基因同源的基因表达水 平最高，而25SrRNA基因表达水平最低。根据上述结果可以推测，适当 控制这些基因在水稻体内的表达水平有可能增强水稻对白叶枯病的抗性。更多还原

【Abstract】 The mutant SL3751 of the partial deletion of the rpfC gene of Xanthomonas oryzae pv. oryzae (Xoo for short) is a potantial biocontrol strain which could induce the rice (Oryzae satira) to produce the resistance to the bacterial blight caused by the wildtype strain 13751. With the eDNA micrroarray technique, eDNA clones of the 10 differential expression gnes of the seeding of the rice Jingang 30 before the tiller were screened, and further verified by Northern hybridization.The foreign cDNA fragments of four of the differential cDNA clones were sequenced.The results of analysis of the nucleiotide sequences displayed the four differentially espressed genes: cytochrome b5 gene,aquaporin gene,25SrRNA gene and a gene homologous to the gene encoding a hypothetical protein in Arabidopsis thaliana.With the exception of cDNA fragment of 25SrRNA gene, cDNA fragments of the rest of three differentially expressed genes were all in full length. As compared with treatments induced by inoculation of 13751 and the control , within the rice induced by inoculation of the strain SL3751 the expression level of the cytochrome b5 gene was lower than that induced by inoculation of the strain 13751 ,but higher than that induced by the control treatment. Expression levels of both the aquaporin gene and the gene homologous to the gene encoding a hypothetical protein in A.thaliana were highest, but the expression level of 25SrRNA gene was lowest. According to the aforementioned results,it might be deduced hat the resistance level of the rice to the bacterial blight caused by Xoo would be enhanced by the proper regulation of the expression level of these genes within the rice.更多还原