蟋蟀卵子发生的显微与超微结构的研究

【作者】 郎冬梅；

【导师】 奚耕思；

【作者基本信息】 陕西师范大学 ， 动物学， 2001， 硕士

【摘要】 本研究以蟋蟀科、北京油葫芦为材料，在光镜水平下，对其卵子发生的过程进行了阶段划分，为三个时期七个阶段。三个时期为：卵母细胞分化期、卵母细胞生长期和卵母细胞的卵黄形成期。 其次，在卵子发生的过程中，对其卵母细胞和滤泡上皮细胞中的DNA和RNA的变化情况进行了普通组化的初步研究；表明在卵子发生的前三个阶段，卵母细胞中RNA和DNA的活动比较活跃，以后便慢慢减弱；而滤泡上皮细胞在卵母细胞卵黄形成期，它的RNA和DNA的合成均较快，因此认为滤泡上皮细胞合成分泌了一些物质参与卵黄蛋白的形成。 最后，对卵母细胞和滤泡上皮细胞超微结构进行了现察，表明滤泡上皮细胞除了对外来物质具筛选作用外，还有加工和合成一些物质运送给卵母细胞，协助其发育的作用。同时对细胞器与卵黄蛋白的关系进行了初步研究，认为卵母细胞内线粒体、高而基体、多泡小体及内吞泡等都直接或间接地参与了卵黄小板的形成与最终成熟。更多还原

【Abstract】 An investigation on ovny of cricket, Teleogrllus emma (OhInachi &Matsuura) is reported in the present paper, which include several aspects. First,the microstructures of preovulatory oocytes and stages of developing oocyteshave been observed by ligh microscope. Three phases and seven stages can becovered in all oocytes growth, and yolk formation period can be identified bytheir special characters in those phases.Secondly, the trends of RNA and DNA in the oocytes and follicular cells aredisplayed by histochemical method during oogenesis, which indicate that RNAand DNA are synthesized by the oocyte obviously at the former three stages.Then their synthese speed slows down. But the synthese of RNA and DNA inthe follicular cell is continuous during vitellogenesis, which may relate toformation of livetin.Last, the ultrastructure of the oocyte and follicular cells have been checkedby electron microscope. According to the observation, the behaviours of thefollicular cell not only relate to filter of the extraeous substance which wanthrough the follicular cell into oocyte, but also process and compose somesubstance for oocyte during vitellogenesis.更多还原