【作者】 刘月环；

【导师】 徐宁迎；

【作者基本信息】 浙江大学 ， 动物遗传育种， 2001， 硕士

【摘要】 本次试验采用微量全血抽提基因组 DNA法和 PCR－RFLP技术分别对大 观山长白种猪，牧场长白、大约克等种猪及绍兴福景达农业有限公司长大 杂交猪的氟烷基因及肠毒素型大肠杆菌(Enterotoxignic E coli，ETEC) F18 抗原受体基因（FUT1基因）的基因型进行测定，计算了基因型频率及相应等 位基因的频率，建立了上述两个基因对猪生产性能效应的固定模型，所测 基因型及其群体生产性能的效应用SPSS软件分析，就FUT1基因与断奶仔 猪腹泻发病率的关系进行了独立性x~2检验，结果如下： 氟烷基因的三种基因型频率在总样本数水平上分别是NN型74．3％、Nn 型24．5％、nn型1．2％，两个等位基因的频率分别是N基因86．5％、n基因 13．5％。其中大观山猪群中三种基因型频率分别是NN型75．9％、Nn型23％、 nn型 1．1％，两个等位基因的频率分别是 N基因 87．4％、n基因 12.6％；牧 场猪群中三种基因型频率分别是NN型67．3％、Nn型31％、nn型1．7％， 两个等位基因的频率分别是 N基因 82．8％、n基因 17．2％。 FUT1基因的三种基因型频率在总样本数水平上分别是GG型81．4％、 AG型 16．6%、AA型 2%，两个等位基因的频率分别是 G基因 89．7%、A基 因10．3％。其中大观山长白猪群中三种基因型频率分别是GG型91．3％、AG 型8．7％、AA型0，两个等位基因的频率分别是G基因95．7％、A基因4．3％； 牧场猪群中三种基因型的频率分别是GG型34．5%、AG型53．4%、AA型 12．1％，两个等位基因的频率分别是G基因61.2％、A基因38．8％；绍兴商 品群中三种基因型的频率分别是 GG型 84．4％、AG型15.6％、AA型 0，两 个等位基因的频率分别是G基因92．2％、A基因7．8％。 断奶仔猪腹泻发病率的分析检验证实发病率与猪本身的遗传敏感基因G 第1页共77页 Thesis ofMaster’s Degree 浙江大学硕士论文：氟烷基因与FUT！基因对猫生产性能效应的研究 有关，而与当时给仔猪饲喂中药“止痢神散”无关。 两个基因位点对猪生产性能效应的研究表明：N基因对总产仔数、20 日龄仔猪成活率、断奶前后仆猪的生长效应显书（P＜0．05），11丛囚X什了腴 厚的效应显著0＜0刀5XG基因在仔猪初生头重、初生窝重上表现出一定 的剂量效应，断奶后的生长速度上效应显著（P＜0刀5），A基因利于胎儿成 活。二基因互作时，氟烷位点显性纯合与 FUT位点杂合的基因型 N’N－AG 在产仔数性状上效应显著o吻刀5），两对等位基因表现出一定的互补效应。 两位点的双杂合子Nn－AG表现出加性效应，总产仔数比基因型Nn－GG总 产仔数多1刀4头（P＜0刀5），产活仔数多1．89头（P＜O．01）。更多还原

【Abstract】 We extract genome DNA by the method which so-called minim wholeporcine blood in this experiment. Then, we determine the alleles and genotype frequencies ofHalothane gene and FUT1 gene (two alpha (1,2)-fucosyltransferase gene) in three groupswine, which include two species lines and one commercial line by PCR-RFLP method.Among of them, one group is Landrace origin (Daguanshan group), another is purebredLandrace, purebred large white (Campus farm group), the commercial line is the crossbred ofLandrace and the large white (Shaoxing group). At the same time, we constfuct two fixedeffect models in order to study on Halothane locus and FUT1 locus and analyze their effectsfor the swine performance. By the SPSS software, we analyze the effect of Halothane andFUT1 locus on some traitS of pigs, and analyze the relationship between FUT1gene and thepiglet’s morbidity by independence X~2 test.The result indicates that three genotype frequencies of the Halothane gene, which isonly determined in two samples (Daguanshan group and campus farm group), theirfrequencies of three genotypes are 74.3%(NN), 24.5%(Nn) and 1.2%(nn). The two allelicfrequencies are 86.5%(N) and 13.5%(n). In the sample of Daguanshan group, the frequenciesof three genotypes are respectively 75.9%(NN), 23%(Nn) and 1.1% (nn), while those ofallele are 87.4%(N) and 12.6%(n). In another sample, campus farm group, the frequencies ofthree genotypes are 67.3%(NN), 31%(Nn) and 1.7%(nn), whiIe its corresponding aIIelicfrequencies are 82.8%(N) and 17.2%(n).As for FUT1 Iocus in three samples are as follow: 81.4%(GG), 16.6%(AG), and2%(AA). The aIleIic frequencies are 89.7%(G) and 10.3%(A) respectively. In Daguangshangroup, three genotypic frequencies of FUT1 locus are 91.3%(GG), 8.7(AG) and 0%(AA)respectively and the allelic frequencies are 95.7%(G) and 4.3%(A). In campus farm gtoup,three genotypic frequencies are 34.5%(GG), 53.4%(AG), and 12.1%(AA) respectively. Itscorresponding allelic frequencies are 61.2% (G ) and 38.8%(A). In the third sample, Shaoxingcommercial group, the genotypic frequencies of FUTl locus are 84.4%(GG), l5.6%(AG),and 0%(AA) respectively. ltS aIlelic frequencies are 92.2o/o(G) and 7.8%(A).The post weaning pigIets diarrhea morbidity analyzing shows that the morbidity is notrelevant to the traditional Chinese medicinaI compound ’Zhilishensan’ which piglet is fedthen, but relevant tO inherit6d susceptibility gene G ofpiglet.We study the effect of the HaIothane and the FUTl gene for tl1e performance of swine.The resultS show that ’N’ gene has a significantly effect (P<0.05) on the total number bom inlitteF, 20d survival number and the groWth-rate before or after weaning in pigIet. ’n’ gene hasa significantly effect (P<0.05) on the backfat. A dose effect-origin from ’G’ gene isdeveloped on the average weight of pigIets bom, the average weight of littCrs, and thesignificantly effect on the groWth-rate after weaning (P<0.05). ’A’ gene seems to in favors ofthe fetus surviving. When we investigate the intOracting effect of tWo loci, we get the resultthat the genotype NN-AG composing of dominant homozygote on Halothane locus andheterorygote on FUTl locus has a significant1y complementny effect on the number born inlitter (P<0.05). The genotype Nn-AG, which are hetCrorygotes on two Ioci, possess additiveeffect, comparing to the genotype Nn-GG on the totaI number born in Iitter and the numberborn alive, the former taking on additive effect is l.04 piglets (P<0.05) and l.89 piglets(P<0.01) respectively更多还原