【作者】 钱叶斌；

【导师】 陶有略；

【作者基本信息】 福建中医学院 ， 中医骨伤科学， 2000， 硕士

【摘要】 目的：研究补阳还五汤对周围神经损伤后脊髓前角运动神经元和脊神经节感觉神经元存活的影响，为临床应用和进一步研究补阳还五汤治疗周围神经损伤提供客观的理论依据。 方法：成年SD大鼠20只，在右侧梨状肌下缘0.5cm处切断坐骨神经，随机分为二组，对照组以生理盐水灌胃，实验组以补阳还五汤灌胃，4周后观察相应节段运动神经元和感觉神经元存活率、神经元胞体直径和面积。 结果：4周后相应节段脊髓前角运动神经元存活率：对照组为59.87％，实验组为69.22％（P＜0.01），脊神经节感觉神经元存活率：对照组为70.99％，实验组为79.13％（P＜0.01）。对照组运动神经元和感觉神经元胞体直径、面积明显小于实验组（P＜0.01）。 结论：补阳还五汤能提高周围神经损伤后脊髓前角运动神经元和脊神经节感觉神经元存活率，减轻神经元胞体萎缩程度，有利于周围神经损伤后神经功能的恢复。更多还原

【Abstract】 Objective: To investigate the effect of buyang huanwu decoction on the survival of the motor neurons of the anterior horn of spinal cord and the sensory neurons in dorsal root ganglion (DRG) after the peripheral nerve injury. Methods: Twenty adult Sprague-Dawley rats were randomly divided into two groups after the right sciatic was transected. Postoperatively, bu-yang huanwu decoction was administered daily to rats of the experimental group. For rats of the conrol group, normal saline was given. By the end of four weeks, the L4,5 spinal region and L4,L5 DRG were dissected out for neuron count and morphological analysis. Results: The survival rate of motor neurons in the experimental group was 69.22% and was statistically significant compared with that in the c-ontrol group, which is 59.87% (P<0.0l). The survival rate of sensory ne-urons was 79.13% in the experimental group, 70.99% in the control group and the difference was statistically significant (P<0.0 1). The diameter and area of motor neurons and sensory neurons were significantly smaller in the control group than those in the experimental group (P<0.01). Conclusion: These results suggest that buyang huanwu decoction can increase the survival rate of neurons and counteract the atrophy of neurons after peripheral nerve injury.更多还原