【作者】 朱宏波；

【导师】 吴宗璞；

【作者基本信息】 东北农业大学 ， 作物遗传育种， 2000， 硕士

【摘要】 本试验采用两个抗SMV、FLS和SCN的品种东农92-8033、东农92-8055，及两个感三种病害的品种丰收22和东农96-84，在隔离网室采用盆栽试验，分别进行单独接种和复合接种三种病害，分别在开花期（R1）和鼓粒期（R5）测定各种生理生化性状，结果表明： 大豆健株叶片中总酚含量、总黄酮含量、PPO活性在开花期和鼓粒期多抗品种与感病品种间的差异不明显。大豆分别接种SMV₁和SCN₃的条件下，叶片中总酚、总黄酮和PPO活性的变化是基本一致的。在开花期，抗病品种叶片中总酚含量增加或变化不大，感病品种降低，总黄酮含量抗病品种增加，而感病品种降低，而且抗病品种接种株总黄酮含量高于感病品种；在鼓粒期，叶片中总酚含量抗感品种均呈现降低趋势，多抗品种总黄酮含量增加，感病品种降低，多抗品种接种株总黄酮含量仍高于感病品种。在开花期，多抗品种PPO活性升高，感病品种降低，在鼓粒期，抗感品种均降低，两个时期多抗品种接种株PPO活性均高于感病品种。 大豆感染FLS₇后，多抗品种总酚含量及多酚氧化酶活性抗感品种表现一致的变化：接种株均低于健株。而总黄酮的含量变化与接种关系不大。在鼓粒期，复合接种两种和三种病害，各接种处理总酚含量、PPO活性抗感品种均明显降低，与同一时期接种单一病害时的变化一致。总黄酮的含量抗病品种增加感病品种降低。 在鼓粒期，大豆健株根系总酚含量、多酚氧化酶活性（?）没有抗感间的差异，而总黄酮含量多抗品种明显高于感病品种。接种SCN₃的情况下，多抗品种根系中总酚含量增加，而感病品种降低；多抗品种总黄酮含量变化很小，感病品种接种株比健株明显增加。PPO活性抗感品种均无明显变化。更多还原

【Abstract】 Two soybean varieties, Dongnong92-8033, Dongnong92-8055, resistant to SMVI, FLS7 and SCN3, and two soybean varieties, Dongnong96-84 and Fengshou22 susceptible to the three diseases, were grown in the net house. They were infected with one, two or three kinds of the diseases. The leaves were taken for biochemical analysis in two periods. The results showed that total phenolics, flavonoids and PPO activity in leaves of healthy soybean had no difference between resistant and susceptible varieties. Changes of total phenolics and flavonoids content and PPO activity in leaves between infected by SMV and SCN were similar. In resistant varieties, total phenolics and flavonoids content and PPO activity of infected soybeans were higher than those of healthy soybeans in Ri. And those of suspected varieties were contrary to resistant varieties. In R5, total phenolics and flavonoids content and PPO activity decreased in all the soybeans. After infected by FLS, the changes of biochemical characters of soybeans had no difference between resistant and susceptible varieties. The changes of the biochemical characters of soybeans after infected with two or three diseases were accord with those of soybeans infected one kind of the diseases. Total phenolics and PPO activity in roots of healthy soybean had no difference between resistant and susceptible varieties. Flavonoids content in roots of healthy soybean of the resistant varieties was much more than those of susceptible varieties. After infected with SCN, in resistant varieties, total phenolics content of infected soybeans was higher than those of the healthy soybeans. After infected with SCN, the changes of flavorioids content of the roots of resistant varieties were not apparent, and that of susceptible varieties increased. The change of PPO activity of both resistant varieties and susceptible varieties were not apparent. Postgraduate: Zhu Hongbo Major: breeding of plants Advisor: Prof. Wu Zongpu更多还原