低磷奶牛红细胞膜超微结构变化的研究

【作者】 徐冰；

【导师】 陈维多；

【作者基本信息】 东北农业大学 ， 兽医内科学， 2000， 硕士

【摘要】 本实验以分组对比的方法对低磷引起的奶牛红细胞膜损伤的超微结构变化进行了研究。对红细胞膜磷脂组份与其变形性、红细胞膜钙依赖中性蛋白酶、Ca²⁺—ATP酶活性、红细胞膜补体蛋白活性、红细胞抗氧化功能、红细胞冰冻蚀刻电镜蛋白形貌进行了检测和观察，其结果如下： 1.低磷组红细胞膜组分发生明显改变，磷脂酰胆碱和磷脂酰丝氨酸显著低于对照组，红细胞变形能力与红细胞膜磷脂酰胆碱呈显著负相关（p＜0.01）。 2.红细胞膜抗自由基主要酶SOD和CAT活性明显低于对照组（p＜0.01、p＜0.05），而MDA含量则显著高于对照组。其含量与红细胞SOD和CAT活性呈极显著正相关，与MDA含量呈极显著负相关。 3.低磷组红细胞膜钙依赖中性蛋白酶活性增高，这说明红细胞膜流动性降低，膜骨架加速水解，失去稳定结构。 4.低磷组的红细胞膜阴离子通透系数增大，说明膜带3蛋白通道发生改变，水分子等不断扩散进入红细胞，导致红细胞体积的膨胀、变形。 5.低磷组奶牛红细胞膜补体蛋白的表达率明显低于对照组（p＜0.01），这表明糖化磷脂酰基锚蛋白（ankyrin）数量减少，使红细胞补体蛋白固定在红细胞膜上的数量减少。容易被补体系统识别，导致溶血。 6.冰冻蚀刻电镜蛋白质形貌学观察，低磷组红细胞膜蛋白微粒分布稀少，个别区域有丛簇状分布。这表明红细胞膜蛋白结构、功能发生了变化。膜骨架蛋白分散，失去稳定结构。更多还原

【Abstract】 The molecular changes of affection to erthrocytic membrane in low phosphorus cows were studied by the method of dividing into groups. The cows were divided into two groups, including low hosphorus (LPG) and health group (HG). The content of phospholipid composition and fluidity, Viscosity and activity of calpain and ca2 AT- Pase of erythrocytic membrane, erthrocytic anti oxidant functional indexes (SOD, CAT, MDA), Scan freeze etching microscopic observation, the expression of mem- brane associated complement regulatory proteins (CRP) D59 were detected . The re- sults are as follows: 1. The content of phospholipid composition in LPG changed significantly: phos- phatidylserine (PS) content in LPG was significantly lower than in HG, fluidity and de- formability of erythrocyte in LPG in comparison with PS were significant negative corre- la tiOn. 1. The anti xidant function of erythrocyte in low phosphorus cows decreased sig- nificantly. . Eerery indexes reflecting the anti oxidant functions of erythrocyte had significant differences between the two groups. There were a significant positive corre- lation between serum phosphorus and erythrocytic SOD activity and CAT activity and a negative correlation between serum phosphorus and erythrocytic MDA content. 2. The thoroughfare coefficient of anion in LPG increased significantly It proved that band III was significantly increased than HG. Water expand in red cell constantly and lead erythrocyte into break. 3. Expression of CD59 on erythrocytic membrane were remarkably decreased in LPG. It shows the quantities of GPI decreased significantly in LPG. Erythrocytic in LPG were to identify by the system of complement regulatory and destroyed by it. 4. Freeze tching microscope scans the proteins of erythrocytic membrane in low phosphorus cows. The glutaraldehyed fixed membrane surface of RBCs. and on manometer range it shows that the proteins of erythrocytic membrane in LPG had great change in destructure and function.更多还原