【摘要】 目的:建立猕猴桃根与猕猴桃藤中化学成分的定性分析方法，系统比较猕猴桃根与猕猴桃藤的化学成分异同。方法:采用液质联用技术，应用负离子扫描模式，获取猕猴桃根与猕猴桃藤样品的高分辨质荷比及离子响应强度信息;利用主成分分析(principal component analysis,PCA)和正交偏最小二乘法-判别分析(orthogonal partial least squares-discriminant analysis,OPLS-DA)对预处理后的数据集进行多变量统计分析，筛选出两者间的差异性成分。结果:猕猴桃根与猕猴桃藤所含化学成分相似，主要为三萜类、黄酮类和苯丙素类化合物，但含量上存在一定差异，筛选得到31个差异成分，猕猴桃根中积雪草酸等三萜类化合物、儿茶素等黄酮类化合物的含量高于猕猴桃藤，秦皮苷、秦皮乙素等5个差异化合物在猕猴桃藤中的含量相对较高。结论:本研究明确了猕猴桃根与猕猴桃藤所含化学成分的异同，猕猴桃根中掺入猕猴桃藤会影响其品质。本研究的开展对于确保猕猴桃根的品质、指导临床用药以及开发猕猴桃根和猕猴桃藤药用资源具有重要意义。更多还原

【Abstract】 Objective: To establish a qualitative analytical method for the chemical constituents in the root and vine of Actinidia chinensis Planch,and to systematically compare the similarities and differences between them.Methods: LC-MS was performed in negative ion mode to acquire high-resolution mass-to-charge ratios and ion intensity data of the samples from the root and vine of Actinidia chinensis Planch. Principal component analysis( PCA) and orthogonal partial least squares-discriminant analysis( OPLS-DA) were applied to the preprocessed dataset to rapidly identify differential components between the two samples. Results: The chemical compositions of root and vine were similar,primarily consisting of triterpenoids,flavonoids and phenylpropanoids,but with certain differences in content. Multivariate analysis identified 31 differential components. The root showed higher contents of triterpenoids( e. g.,asiatic acid) and flavonoids( e. g.,catechin) than the vine,while five differential compounds,including fraxin and aesculetin,were relatively more abundant in the vine. Conclusion: This study clarifies the similarities and differences in chemical composition between the root and vine of Actinidia chinensis Planch. The admixture of vine into the root was found to compromise its quality. This study provides significant implications for ensuring the quality of root of Actinidia chinensis Planch.,guiding clinical medication,and facilitating the medicinal development of both root and vine resources.更多还原