【摘要】 植物线虫因受寄主细胞壁的阻隔，传统分离技术存在效率低、仅能分离自由迁移虫态等不足。本研究通过优化纤维素酶与果胶酶的比例和处理时间，建立了一种新型酶解分离技术。结果表明，纤维素酶与果胶酶的最佳比例为1∶30，最佳处理时间为6 h；该技术通过特异性裂解植物组织，释放内寄生线虫，可同步分离死虫与活虫，实现不同发育阶段虫体的大量富集，将分离时间从24 h缩短至6 h。该方法可显著提升口岸检疫时效，也可为植物线虫分子生物学研究提供重要技术支持。更多还原

【Abstract】 Due to the barrier of the host cell wall,traditional isolation techniques for plant nematodes have shortcomings such as low efficiency and the ability to only separate freely migrating insect forms.A novel enzymatic isolation protocol was established by systematically optimizing the cellulase:pectinase ratio and incubation time. The optimal conditions were determined to be a 1∶30 cellulase:pectinase ratio and 6 h of incubation. By selectively lysing plant tissues,the technology simultaneously releases and separates both live and dead endoparasitic nematodes,enabling large-scale enrichment of all developmental stages while reducing the total processing time from 24 h to 6 h. This method can not only significantly improve the efficiency of port quarantine but also provide important technical support for molecular biology research on plant nematodes.更多还原