【摘要】 为筛选出对双斑长跗萤叶甲Monolepta hieroglyphica有吸引作用的寄主植物，连续两年在田间种植15科60种植物，采用直接观察法调查试验小区中害虫及天敌种类和数量，并用固相微萃取法(SPME)对双斑长跗萤叶甲取食偏好寄主植物挥发物进行采集，单四极杆气相色谱质谱联用仪(GC-MS)分析鉴定。结果表明：60种植物中苋Amaranthus tricolor上的刺吸式口器害虫数量最多，占所有刺吸式口器害虫数量的3.98%;番茄Solanum lycopersicum上的咀嚼式口器害虫数量最多，占所有咀嚼式口器害虫数量的5.51%;玉米Zea mays(好再来超甜糯水果玉米)上的天敌类群数量最多，占9.85%。害虫优势度最大的是小绿叶蝉Empoasca flavescens,其次是双斑长跗萤叶甲，天敌最大优势种各有不同。双斑长跗萤叶甲有14个偏好寄主植物，分别为：大豆Glycine max(‘吉育47’)、玉米(好再来超甜糯水果玉米)、大豆(黑豆)、咖啡黄葵Abelmoschus esculentus、玉米(‘吉农糯7号’)、大豆(‘吉育308’)、油麦菜Lactuca sativa var.asparagina、红秋葵Hibiscus coccineus、白菜Brassica rapa var.glabra(顶心黄三号)、蕹菜Ipomoea aquatica、辣椒Capsicum annuum(青线椒)、玉米(好再来三号)、稗Echinochloa crus-galli、苋。对大豆(‘吉育47’)、大豆(黑豆)、油麦菜、蕹菜植物挥发物进行收集鉴定，筛选出5种共有挥发物：2-己烯醛、苯甲酸、辛酸、癸醛、反式-β-紫罗酮。本试验结果不仅为害虫田间绿色防控提供了新思路，还为双斑长跗萤叶甲植物源引诱剂的研发奠定了理论基础，有望在减少化学农药使用、保护生态环境方面发挥重要作用。更多还原

【Abstract】 To screen host plants attractive to Monolepta hieroglyphica, 60 plant species from 15 families were planted in the fields for two consecutive years. Pest and natural enemy species and their abundances in experimental plots were surveyed through direct observation. Volatile compounds emitted from the preferred host plants of M.hieroglyphica were collected using solid-phase microextraction(SPME) and identified using gas chromatography-mass spectrometry(GC-MS) with a single quadrupole detector. The results indicated that among the 60 plant species, Amaranthus tricolor harbored the highest abundance of piercing-sucking mouthpart insects, accounting for 3.98% of the total piercing-sucking mouthpart insect population; Solanum lycopersicum had the highest abundance of chewing mouthpart insects, representing 5.51% of the total chewing mouthpart insect population; Zea mays(Haozailai super sweet and glutinous fruit maize) supported the highest abundance of natural enemies, representing 9.85% of the total numbers. Empoasca flavescens was the most dominant pest group, followed by M.hieroglyphica, while the dominant natural enemy species varied among host plants. Fourteen plant species were identified as preferred host plants of M.hieroglyphica, including Glycine max(‘Jiyu 47’), Zea mays(Haozailai super sweet and glutinous fruit maize), G.max(black soybean), Abelmoschus esculentus, Z.mays(‘Jinongnuo 7’), G.max(‘Jiyu 308’), Lactuca sativa var. asparagina, Hibiscus coccineus, Brassica rapa var. glabra(Dingxinhuang 3), Ipomoea aquatica, Capsicum annuum(Qingxian jiao), Z.mays(Haozailai 3), Echinochloa crus-galli and A.tricolor. Volatile compounds from G.max(‘Jiyu 47’), G.max(black soybean), L.sativa var asparagina and Ip.aquatica were further analyzed, and five common volatiles compounds were identified: 2-hexenal, benzoic acid, octanoic acid, decanal and trans-β-ionone. This study provides a theoretical foundation for green pest management in the field and establish a theoretical basis for the development of attractants for M.hieroglyphica, with potential benefits for reducing chemical pesticide use and protecting the ecological environment.更多还原