【摘要】 为了探究槐花散露对大鼠试验性结肠炎的作用，本试验将无特定病原体（SPF）级SD大鼠随机分为对照（CON）组、模型（DSS）组和槐花散露灌胃（DSS+HHSL）组，应用葡聚糖硫酸钠（DSS）构建大鼠试验性结肠炎模型，评估各组大鼠整体状态和结肠炎疾病活动指数（DAI），并进行结肠组织病理学观察和结肠黏膜菌群16S rRNA测序，探究槐花散露对试验性结肠炎大鼠的作用，并通过高效液相色谱（HPLC）检测槐花散露的主要成分。结果显示，成功建立大鼠试验性结肠炎模型，槐花散露能够显著降低结肠炎大鼠DAI(P<0.05)，缓解结肠损伤，减轻局部炎症反应，调节结肠黏膜菌群结构，抑制病原菌丰度，增加益生菌丰度。槐花散露供试品芦丁含量为0.005 8 mg/mL，柚皮苷含量为0.005 1 mg/mL。结果表明，槐花散露能有效改善大鼠试验性结肠炎。本试验为槐花散露的药用价值研究提供了一定参考依据。更多还原

【Abstract】 To investigate the effects of Huaihua San Lu(HHSL) on experimental colitis in rats, specific pathogen-free(SPF) Sprague-Dawley(SD) rats were randomly assigned to Control(CON) group, Model(DSS) group, and HHSL treatment group(DSS+HHSL). Experimental colitis was induced using dextran sulfate sodium(DSS). The overall condition and disease activity index(DAI) of colitis were evaluated in each group. In addition, histopathological examination of the colon and 16S rRNA sequencing analysis of colonic mucosal microbiota were performed to assess the effects of HHSL on DSS-induced colitis. The main components of HHSL were determined by high-performance liquid chromatography(HPLC). The results showed that the rat model of experimental colitis was successfully established. HHSL significantly reduced the DAI score in colitis rats(P < 0. 05), alleviated colonic injury, decreased local inflammatory responses, modulated the structure of the colonic mucosal microbiota, suppressed the abundance of pathogenic bacteria, and increased the abundance of beneficial bacteria. The contents of rutin and naringin in the HHSL were 0. 005 8 mg/mL and 0. 005 1 mg/mL, respectively. These findings indicate that HHSL can effectively ameliorate experimental colitis in rats and provide a reference for further studies on its medicinal value.更多还原