【摘要】 A型塞内卡病毒(senecavirus A,SVA)是小RNA病毒科、塞内卡病毒属的唯一成员，它所引起的感染严重威胁全球养猪业的健康发展，其致病机制目前尚不完全清楚。为探究SVA感染对溶酶体相关蛋白表达及其功能的影响，本研究利用蛋白组学方法、GO和KEGG富集法检测与分析SVA感染BHK-21细胞后溶酶体蛋白的差异表达，及其相关信号通路。结果显示，SVA感染BHK-21细胞后溶酶体相关膜蛋白1(LAMP1)的表达显著增加，表明SVA激活宿主细胞溶酶体的生物发生。蛋白组学分析鉴定出451个差异表达蛋白(DEPs),其中101个上调，350个下调。进一步对这些蛋白的定位分析发现，34个参与细胞自噬、泛素化、囊泡运输等过程的溶酶体相关蛋白表达水平呈现显著变化。GO和KEGG富集法分析结果显示，免疫相关通路如抗原处理与递呈、NOD样受体信号通路和RIG-I受体信号通路被激活。本研究结果有助于揭示溶酶体在SVA复制过程中发挥的作用，并为SVA的防制提供新的靶点和干预思路。更多还原

【Abstract】 Senecavirus A(SVA),the sole member of the genus Senecavirus within the family Picornaviridae,poses a significant threat to the global swine industry.The pathogenic mechanisms of SVA remain incompletely understood.To investigate the impact of SVA infection on lysosomal protein expression and function,we performed proteomic profiling coupled with GO and KEGG enrichment analyses to identify differentially expressed lysosomal proteins and their associated pathways in infected BHK-21 cells.The results showed that SVA infection activated the lysosomal biogenesis of BHK-21 cells,as evidenced by an increased expression of lysosome-associated membrane protein 1(LAMP 1).Proteomic analysis identified 451 differentially expressed proteins(DEPs),among which 101 were upregulated and 350 were downregulated.Among these,34 lysosome-localized proteins displayed significant changes in expression and are involved in processes such as autophagy,ubiquitination,and vesicular trafficking.GO and KEGG enrichment analyses indicated the activation of immune-related pathways,including antigen processing and presentation,the NODlike receptor signaling pathway,and the RIG-I-like receptor signaling pathway during viral infection.Our results not only shed light on the mechanistic role of lysosomes in SVA replication,but also identify potential new targets and ideas for its prevention and control.更多还原