【摘要】 目的 明确调脂通脉颗粒的化学组成及其入血、入肝成分，揭示其潜在的药效物质基础。方法 采用超高效液相色谱-高分辨质谱(UPLC-HRMS)技术对调脂通脉颗粒进行全面化学成分分析，通过保留时间、精确质荷比、MS/MS碎片信息及对照品质谱比对，系统鉴定制剂中的体内外化学成分；并结合灌胃给药后大鼠血浆及肝脏样本，筛选入血和入肝原形成分。结果 共鉴定出143种化学成分，主要包括黄酮类、萜类和醌类等。在灌胃给药后，大鼠血浆中检测到87个入血原形成分，肝脏中检测到57个入肝原形成分，部分成分在两者中同时存在，提示其可能为活性物质基础。结论 本研究运用UPLC-HRMS技术结合血清药物化学策略，系统揭示了调脂通脉颗粒的化学成分及体内转运特征，为其作用机制研究及后续开发提供了实验依据。更多还原

【Abstract】 Objective To characterize the chemical constituents of Tiaozhi Tongmai granules and identify the absorbed components in the blood and the liver,thereby elucidating the potential pharmacologically active substances.Methods Ultra-performance liquid chromatography coupled with high-resolution mass spectrometry (UPLC-HRMS) was used to comprehensively profile the chemical constituents of Tiaozhi Tongmai granules.The components were identified by the retention time,mass-to-charge ratio,MS/MS fragmentation patterns,and comparison with reference standards and spectral libraries.After oral administration,prototype compounds present in serum and liver tissues of rats were screened to determine the in vitro and in vivo components.Results Totally 143 compounds were identified from the extract,mainly including flavonoids,terpenoids,and quinones.Among them,87 prototype compounds were detected in rat serum and 57 in liver tissue.Several components were found in both matrices,suggesting their potential relevance as bioactive substances.Conclusion This study,through UPLC-HRMS combined with serum pharmacochemistry strategies,reveals the chemical composition of Tiaozhi Tongmai granules and their in vivo distribution characteristics,providing an experimental basis for further pharmacological and mechanistic research.更多还原