【摘要】 采用常规检测方法检测南阳市西峡县奎文村（KW）、屈原岗村（QYG）、王营村（WY）猕猴桃根际土壤样品的基本理化指标，采用Illumina Mi Seq高通量测序技术分析其微生物群落多样性，并分析两者间的相关性。结果表明，3个样品的pH、有效磷含量、速效钾含量、碱解氮含量差异显著（P<0.05）。3个样品细菌群落多样性及物种丰度无显著差异（P>0.05）,QYG样品真菌群落多样性最高，KW样品最低，但3个样品真菌群落的物种丰度无显著差异（P>0.05）。从3个样品中共注释到51个细菌门和16个真菌门，包括16个优势细菌门和6个优势真菌门，其中芽孢杆菌门（Bacillota）和放线菌门（Actinomycetota）作为潜在的猕猴桃溃疡病生防细菌，在3个样品中均为优势菌门。土壤理化性质对猕猴桃根际土壤微生物群落分布具有显著影响，其中，芽孢杆菌门与速效钾含量呈极显著负相关（P<0.01），放线菌门与速效钾含量呈显著正相关（P<0.05），与p H呈显著负相关（P<0.05）;pH与罗兹菌门（Rozellomycota）呈高度显著正相关（P<0.001）。该研究结果为猕猴桃溃疡病生防细菌的筛选鉴定了基础。更多还原

【Abstract】 The basic physicochemical indicators of the rhizsphere soil samples of kiwifruit from Kuiwen Village(KW), Quyuan Gang Village(QYG),and Wangying Village(WY) in Xixia County, Nanyang City were detected by conventional detection methods. The microbial community diversity was analyzed by Illumina Mi Seq high-throughput sequencing technology, and the correlation was analyzed. The results showed that there were significant differences in pH, available phosphorus content, available potassium content and alkali-hydrolyzable nitrogen content among the three samples(P<0.05). There was no significant difference in the bacterial community diversity and species abundance among the three samples(P>0.05). The fungal community diversity of the QYG sample was the highest, while that of the KW sample was the lowest. However, there was no significant difference in the species abundance of the fungal community among the three samples(P>0.05). A total of 51 bacterial phyla and 16 fungal phyla were annotated from the 3 samples, including 16 dominant bacterial phyla and 6 dominant fungal phyla. Among them, Bacillota and Actinomycetota, as potential pathogenic control bacteria for kiwifruit canker, were all dominant phylum in the 3 samples. The physicochemical properties of the soil had a significant impact on the distribution of microbial communities in the rhizosphere of kiwifruit. Among them, the Bacillota was extremely significantly negatively correlated with the content of available potassium(P<0.01), and the Actinomycetota was significantly positively correlated with the content of available potassium(P<0.05), and was significantly negatively correlated with pH(P<0.05). pH was highly extremely significantly positively correlated with the Rozellomycota(P<0.001). The research results laid a foundation for the screening and identification of bacterium that could prevent kiwifruit canker disease.更多还原