【摘要】 为探究酒厂污泥资源化利用途径及其细菌群落结构演变规律，该研究以酒厂污泥为主料进行35 d的超高温好氧堆肥中试实验，测定不同阶段堆肥的理化指标及细菌群落多样性，并对其进行相关性分析。结果表明，堆肥后样品鲜质量降低50.70%，总氮、铵态氮含量分别降低至3.93%、6.71 g/kg，硝态氮、胡敏酸含量、胡敏酸与富里酸比值及种子发芽指数分别提高至327.46 mg/kg、59.24 g/kg、2.73、108.90%，说明在中试规模下的堆肥减量化、无害化效果显著。堆肥过程中细菌菌群丰富度降低，多样性增加，群落结构发生较大演变，在门水平上，优势菌门由变形菌门（Proteobacteria）逐渐演替为厚壁菌门（Firmicutes）；在属水平上，优势菌属由嗜冷杆菌属（Psychrobacter）和不动杆菌属（Acinetobacter）逐渐演替为芽孢杆菌属（Bacillus）和直丝菌属（Planifilum）。芽孢杆菌属、八叠球菌属（Sporosarcina）与堆肥温度极显著正相关（P<0.01），直丝菌属、糖单孢菌属（Saccharomonospora）等与种子发芽指数和胡敏酸含量极显著正相关（P<0.01），嗜冷杆菌属、不动杆菌属等与富里酸含量极显著正相关（P<0.01），与胡敏酸含量和种子发芽指数极显著负相关（P<0.01）。更多还原

【Abstract】 To investigate the way of resource utilization of distillery sludge and the evolution rules of bacterial community structure, an ultra-high temperature aerobic composting pilot experiment for 35 d was conducted using distillery sludge as main raw materials, the physicochemical indicators and bacterial community diversity of compost at different stages were determined, and their correlations were analyzed. The results showed that after composting, the fresh mass of the samples decreased by 50.70%, the total nitrogen and ammonium nitrogen content decreased to 3.93% and 6.71 g/kg,respectively, the nitrate nitrogen, humic acid content, humic acid and fulvic acid ratio, and seed germination index increased to 327.46 mg/kg, 59.24 g/kg,2.73 and 108.90%, respectively, which indicated the effect of composting reduction and harmless treatment were significant in the pilot scale. During the composting process, the richness of bacterial community decreased, the diversity increased, and the bacterial community structure underwent significant evolution. At the phylum level, the dominant phylum gradually evolved from Proteobacteria to Firmicutes; At the genus level, the dominant bacterial genera gradually evolved from Psychobacter and Acinetobacter to Bacillus and Planifilum. Correlation analysis showed that Bacillus and Sporosarcina were significantly positively correlated with compost temperature(P<0.01), Planifilum and Saccharomonospora were significantly positively correlated with seed germination indexes and humic acid contents(P<0.01), Psychrobacter and Acinetobacter were significantly positively correlated with fulvic acid contents(P<0.01), and significantly negatively correlated with humic acid contents and seed germination indexes(P<0.01).更多还原