【摘要】 为定向提高烤烟品种渠首1号对烟草花叶病毒（tobacco mosaic virus,TMV）的抗性，以渠首1号为改良材料，以TMV免疫品种Va770为供体亲本，通过杂交、回交，利用分子标记结合表型筛选得到TMV免疫的纯合株系TMV-3。大田鉴定结果表明TMV-3农艺性状与渠首1号基本保持一致；接种鉴定结果表明TMV-3对TMV的抗性表现为免疫，TMV接种后的活性氧积累量少于渠首1号，叶片中的TMV正调控基因NtRAR1、NtNTF4、NtPR1 a、NtHAK1、NtSGT1的相对表达量均高于渠首1号，负调控基因TMV-CP相对表达量低于渠首1号，黑胫病抗性有所下降。本研究表明，渠首1号定向改良品系TMV-3对TMV免疫，其他性状与渠首1号基本保持一致，为渠首1号病毒病抗性的定向改良提供了材料基础。更多还原

【Abstract】 In order to targeted improve the resistance of flue-cured tobacco variety Qushou 1 to tobacco mosaic virus(TMV),Qushou 1 was used as the improved material,and the TMV-immune variety Va770 was used as the donor parent. Through hybridization,backcrossing,molecular markers combined with phenotypic screening,TMV-immune homozygous lines TMV-3 were obtained. The agronomic traits of TMV-3 were basically consistent with Qushou 1. Inoculation identification results indicated that the resistance of TMV-3 was immune to TMV. After TMV treatment,compared with Qushou 1,TMV-3 accumulated less reactive oxygen species,and the expression of TMV positive regulatory genes NtRAR1,NtNTF4,NtPR1 a,NtHAK1 and NtSGT1 were higher,negative regulatory gene TMV-CP was lower in leaves. In addition,the resistance of TMV-3 to black shank decreased. This study demonstrated that TMV-3,Qushou 1 directional improved line,was immune to TMV,and other traits were basically consistent with Qushou 1,which provided a material basis for the targeted improvement of virus disease resistance of Qushou 1.更多还原