【摘要】 目的 研究千金子制霜前后天然多元微乳对人肝HepaRG细胞脂滴、氧化应激和线粒体的调控作用。方法 将HepaRG细胞分为对照组、千金子天然多元微乳组、千金子霜天然多元微乳组，油红O染色观察细胞脂滴积累;试剂盒法检测细胞活性氧(ROS)、超氧化歧化酶(SOD)、过氧化氢酶(CAT)、丙二醛(MDA)、谷胱甘肽(GSH)、乳酸脱氢酶(LDH)、谷丙转氨酶(ALT)、谷草转氨酶(AST)水平; Western blot检测细胞中线粒体融合蛋白2(Mfn2)、视神经萎缩蛋白1(OPA1)、线粒体动力相关蛋白1(Drp1)蛋白表达。结果 与对照组相比，千金子天然多元微乳可以显著促进HepaRG细胞中脂滴蓄积，提高细胞中ROS、MDA、ALT、AST、LDH水平，抑制细胞中SOD、CAT、GSH水平(P<0.05,P<0.01)，导致HepaRG细胞线粒体融合/分裂失衡(P<0.01);同一质量浓度下，与千金子天然多元微乳相比，千金子霜天然多元微乳对HepaRG细胞脂滴蓄积、氧化应激损伤和线粒体融合/分裂失衡的影响均明显减弱(P<0.05,P<0.01)。结论 千金子制霜后形成的天然多元微乳通过脂滴诱导细胞氧化应激和线粒体融合/分裂失衡的作用明显减弱，可以有效减轻千金子天然多元微乳对人肝HepaRG细胞的毒性作用。更多还原

【Abstract】 OBJECTIVE To study the effects of natural microemulsion from Euphorbia Semen and Euphorbia Semen Pulveratum on lipid droplets,oxidative stress and mitochondria in human hepatic HepaRG cells.METHODS HepaRG cells were divided into control group,Euphorbia Semen natural microemulsion group and Euphorbia Semen Pulveratum natural microemulsion group.Oil red O staining was applied to observe the accumulation of cellular lipid droplets.The levels of reactive oxygen species(ROS),superoxide dismutase(SOD),catalase(CAT),malondialdehyde(MDA),glutathione(GSH),lactate dehydrogenase(LDH),alanine aminotransferase(ALT) and aspartate aminotransferase(AST) were detected using kit assay.The protein expressions of mitofusin 2(Mfn2),optic atrophy 1(OPA1) and dynamin-related protein 1(Drp1) in HepaRG cells were determined by Western blot.RESULTS Compared with the control group,Euphorbia Semen natural microemulsion significantly promoted lipid droplet accumulation in HepaRG cells,increased the levels of ROS,MDA,ALT,AST,and LDH,as well as inhibited cellular levels of SOD,CAT,and GSH(P<0.05,P<0.01),resulting in mitochondrial fusion/disintegration imbalance of HepaRG cells(P<0.01).However,the effects of Euphorbia Semen Pulveratum natural microemulsion on lipid droplet accumulation,oxidative stress injury,and mitochondrial fusion/disintegration imbalance in HepaRG cells were significantly attenuated compared with those of Euphorbiae Semen natural microemulsion at the same dose(P<0.05,P<0.01).CONCLUSION Euphorbia Semen Pulveratum natural microemulsion induced cellular oxidative stress and mitochondrial fusion/disintegration imbalance through lipid droplets is significantly attenuated compared with Euphorbia Semen natural microemulsion,which could effectively attenuate the toxic effects of Euphorbia Semen natural microemulsion on HepaRG cells.更多还原