【摘要】 目的 分析木姜叶柯叶的入血、入脑成分，为明确其防治中枢神经系统疾病的药效物质基础提供参考。方法 制备木姜叶柯叶醇提物以及灌胃液、灌流液。以大鼠为研究对象，通过在体肠灌流法和封闭肠环法制备肠壁代谢、肠道菌代谢、肝代谢全血样本，通过灌胃给药方法制备综合代谢全血样本和脑组织、脑脊液样本。采用超高效液相色谱-高分辨质谱技术，分析鉴定木姜叶柯叶的化学成分及其在大鼠体内的入血及入脑成分。结果 从木姜叶柯叶中共鉴定出66个化学成分，主要为黄酮类、有机酸等。从肠壁代谢、肠道菌代谢、肝代谢与综合代谢全血样本中分别鉴定出16、13、11、5个原型成分，从脑组织样本中鉴定出4个原型成分，从脑脊液样本中鉴定出9个原型成分，其中根皮苷、三叶苷、根皮素-2-O-丙二酰己糖苷、根皮素为各样本的共有成分。结论 木姜叶柯叶入血、入脑原型成分中的根皮苷、三叶苷、根皮素等可能是其治疗中枢神经系统疾病的药效物质基础。更多还原

【Abstract】 OBJECTIVE To analyze the prototype components absorbed into blood and brain of Lithocarpus litseifolius leaves, so as to provide a reference for clarifying the pharmacological material basis of its prevention and treatment of central nervous system diseases. METHODS The ethanol extract of L. litseifolius leaves, as well as the gastric lavage fluid and perfusion solution were prepared. Using rats as subjects, plasma samples of intestinal wall metabolism, intestinal flora metabolism and hepatic metabolism were prepared via in situ intestinal perfusion and closed intestinal loop method; while comprehensive metabolic plasma samples, brain tissue samples, and cerebrospinal fluid samples were collected after intragastric administration. UPLC-HRMS technology was utilized to analyze and identify chemical components and prototype components absorbed into blood and brain of L. litseifolius leaves. RESULTS A total of 66 chemical constituents were identified in L. litseifolius leaves, primarily consisting of flavonoids, organic acids, and others. A total of 16, 13, 11, and 5 prototype components were identified in intestinal wall metabolism, intestinal flora metabolism, hepatic metabolism, and comprehensive metabolic plasma samples, respectively. Additionally, 4 prototype components were detected in brain tissue and 9 in cerebrospinal fluid. Phloridzin, trilobatin, phloretin-2-O-malonyl hexoside, and phloretin were identified as common components across all sample types. CONCLUSIONS Prototype components absorbed into blood and brain of L. litseifolius leaves, such as phloridzin, trilobatin, phloretin, and other components may serve as the pharmacological material basis for their therapeutic effects on central nervous system diseases.更多还原