【摘要】 采用酶预处理结合无溶剂微波萃取(EP-SFME)法提取鲜白芷挥发油，以挥发油得率为评价指标，在单因素实验的基础上通过响应面实验对挥发油提取工艺进行优化。通过GC-MS结合NIST20.L标准谱库检索对其化学成分进行鉴定，并研究挥发油清除DPPH自由基、ABTS自由基、羟基自由基、超氧阴离子自由基的能力。结果表明，最佳提取工艺为微波功率420 W、微波萃取时间40 min、复合酶浓度1.5%、酶解时间2 h、复合酶比例(纤维素酶∶果胶酶)2∶1,在此条件下挥发油得率为(0.27±0.08)%,水蒸汽蒸馏(HD)法和无溶剂微波萃取(SFME)法分别提高47.92%和17.84%。白芷挥发油中共鉴定出67种成分，主要是月桂醇，其次是十四醇、次亚麻酸甲酯、油醇、棕榈酸甲酯、9,12-十八碳二烯酸丁酯、β-榄香烯、γ-榄香烯。3种方法获得的挥发油均具有良好的抗氧化活性，且EP-SFME法优于SFME法和HD法。更多还原

【Abstract】 The volatile oil is extracted from fresh Angelica dahurica by enzyme pretreatment combined with solvent-free microwave extraction(EP-SFME) method. With the yield of volatile oil as the evaluation index, the extraction process of volatile oil is optimized through response surface experiment on the basis of single factor experiment. The chemical components are identified by GC-MS combined with NIST20.L standard spectral library retrieval, and the scavenging capacity of the volatile oil on DPPH radicals, ABTS radicals, hydroxyl radicals and superoxide anion radicals is studied. The results show that the optimal extraction process is microwave power of 420 W, microwave extraction time of 40 min, complex enzyme concentration of 1.5%, enzymatic hydrolysis time of 2 h, and the ratio of complex enzymes(cellulase∶pectinase) of 2∶1. Under these conditions, the yield of volatile oil is(0.27±0.08)%, which is 47.92% and 17.84% higher than that obtained by steam distillation(HD) method and solvent-free microwave extraction(SFME) method respectively. A total of 67 components are identified in the volatile oil from Angelica dahurica, mainly including lauryl alcohol, followed by tetradecanol, methyl linolenate, oleyl alcohol, methyl palmitate, 9,12-octadecadienoic acid butyl ester, β-elemene, γ-elemene and so on. The volatile oils obtained by the three methods all exhibit good antioxidant activities, and EP-SFME method is superior to SFME method and HD method.更多还原