【摘要】 苏木精-伊红（HE）染色是病理诊断中最基础、应用最广泛的常规染色方法，几乎适用于各类动物组织标本，染色质量直接决定病理切片的诊断价值。随着临床病理实验室自动化水平不断提升，全自动HE染色机已成为主流设备，染色程序的合理调试，也成为衔接设备性能与染色效果的关键环节。本文从全自动HE染色机的工作原理入手，系统分析影响染色质量的关键因素，围绕上机前预调试校准、核心染色步骤分步优化、特殊疑难样本适配调整三个方面，构建标准化调试流程；同时建立主观判读与客观指标相结合的双重评估体系，总结常见染色异常问题的针对性解决方案，并对未来智能化、自动化调试技术的发展趋势进行展望。研究旨在为病理实验室提供一套可直接应用的HE染色程序标准化调试方案，提升染色稳定性与诊断准确性，为临床病理技术规范化建设提供实用参考。更多还原

【Abstract】 Hematoxylin eosin(HE) staining is the most basic and commonly used staining technique, applicable to any animal tissue, and its staining quality plays a decisive role in the diagnostic value of pathological sections. In current clinical pathology laboratories, the application of fully automatic staining machines is becoming increasingly common, and program debugging has become a core link in connecting equipment performance and staining quality. This article starts from the working principle of the fully automatic HE staining machine, deeply analyzes the core factors that affect the staining effect, and then outlines the standardized debugging ideas from three dimensions: pre debugging calibration, step-by-step optimization of core processes, and special sample adaptation; Simultaneously constructing a “subjective objective” dual evaluation system, summarizing the debugging and response methods for common dyeing problems, and looking forward to the development direction of intelligent debugging technology. This study aims to develop a practical and fully automated HE staining program debugging framework for pathology laboratories, helping to improve the stability of staining quality and the accuracy of pathological diagnosis, and providing practical reference for the standardization of clinical pathology technology.更多还原