【摘要】 目的 研究黑龙江产麻叶千里光化学成分及其体外抗炎活性。方法 运用HP-20大孔树脂、硅胶、ODS及制备型HPLC进行分离纯化，根据理化性质与波谱数据解析所得化合物的结构。采用RAW264.7模型评价体外抗炎活性。结果 从中分离得到29个化合物，分别鉴定为脱落酸(1)、(6S,9R)-长寿花糖苷(2)、(6R,9R)-3-oxo-α-ionol-O-β-D-glucopyranoside (3)、(Z)-4-[3′-(β-D-glucopyranosyloxy)butylidene]-3,5,5-trimethyl-2-cyclohexen-1-one (4)、akequintoside D (5)、saniculamoid D (6)、二氢猕猴糖内酯(7)、刺参酮(8)、款冬酮(9)、诺卡酮(10)、1β,6α-dihydroxyeudesma-4(15)-ene (11)、4β,10α-dihydroxy-guai-6-ene (12)、莪术醇(13)、purpureaterpene D (14)、蜂斗菜内酯A (15)、白术内酯I (16)、柚皮素(17)、苜蓿素(18)、槲皮素-3-半乳吡喃糖苷(19)、槲皮素3-O-β-D-葡萄糖醛酸苷(20)、山柰酚-3-O-β-D-葡萄糖苷(21)、鼠李素-3-O-洋槐糖苷(22)、山柰酚-3-O-β-D-葡萄糖基-(1→2)-β-D-半乳吡喃糖苷(23)、山柰酚-3-O-β-D-葡萄糖基-(1→2)-β-D-葡萄糖苷(24)、山柰酚-3-O-β-D-芸香糖苷(25)、9(S),12(S),13(S)-trihydroxyoctadeca-10(E),15(Z)-dienoic acid (26)、9,12,13-三羟基-10,15-十八碳烯酸甲酯(27)、5-羟基己酸-4-内酯(28)、齐墩果酸(29)。化合物3、7、15、23、25对NO的相对抑制率为(32.5±2.36)%～(34.2±6.32)%。结论 化合物12、20、23、26、27为首次从菊科中分离得到，22为首次从千里光属中分离得到，3、6～11、13～18、23～24为首次从麻叶千里光中分离得到。化合物3、7、15、23、25具有体外抗炎活性。更多还原

【Abstract】 AIM To study the chemical constituents of Senecio cannabifolius Less. from Heilongjiang province and their in vitro anti-inflammatory activity.METHODS Separation and purification were performed using HP-20 macroporous resin, silica gel, ODS and preparative HPLC, the structures of the isolated compounds were then determined via physicochemical and spectroscopic analyses. The in vitro anti-inflammatory activity was evaluated by RAW264.7 model.RESULTS Twenty-nine compounds were isolated and identified as abscisic acid(1),(6S,9R)-roseoside(2),(6R,9R)-3-oxo-α-ionol-O-β-D-glucopyranoside(3),(Z)-4-[3′-(β-D-glucopyranosyloxy)butylidene]-3,5,5-trimethyl-2-cyclohexen-1-one(4), akequintoside D(5), saniculamoid D(6), dihydroactinidiolide(7), oplopanone(8), tussilagone(9), nootkatone(10), 1β,6α-dihydroxyeudesma-4(15)-ene(11), 4β,10α-dihydroxy-guai-6-ene(12), curcumol(13), purpureaterpene D(14), bakkenolide A(15), atractylenolide I(16), naringenin(17), tricin(18), quercetin 3-galactoside(19), quercetin 3-O-β-D-glucuronide(20), kaempferol 3-O-β-D-glucoside(21), rhamnetin-3-O-robinobioside(22), kaempferol 3-O-β-D-glucosyl(1→2)-β-D-galactoside(23), kaempferol 3-O-β-D-glucosyl(1→2)-β-D-glucoside(24), kaempferol-3-O-β-D-rutinoside(25), 9(S),12(S),13(S)-trihydroxyoctadeca-10(E),15(Z)-dienoic acid(26), methyl-9,12,13-trihydroxyoctadeca-10,15-dienoate(27), 5-hydroxyhexan-4-olide(28), oleanolic acid(29). The relative inhibition rates of compounds 3, 7, 15, 23 and 25 on NO were(32.5±2.36)%-(34.2±6.32)%.CONCLUSION Compounds 12, 20, 23, 26, 27 are isolated from Asteraceae family for the first time, 22 is first isolated from Senecio genus, and 3, 6-11, 13-18, 23-24 are first isolated from S. cannabifolius. Compounds 3, 7, 15, 23 and 25 have in vitro anti-inflammatory activity.更多还原