【摘要】 为了解香格里拉市猪链球菌2型的流行情况，采用ELISA、细菌分离培养、生化鉴定及PCR方法，对从香格里拉市11个乡镇采集的504份藏香猪血清及鼻拭子样本进行检测分析。结果表明，猪链球菌2型抗体阳性率为29.76%；从504份样本中分离鉴定出18株猪链球菌2型，它们对22种抗生素呈现不同程度耐药性且均存在多重耐药性，对四环素类、大环内酯类和磺胺类抗生素的耐药性较强。耐药基因检测显示，大环内酯类耐药基因mefA、msrD、ermA、ermB的检出率分别为44.44%、16.67%、100%和22.22%，四环素类耐药基因tetM、tetO、tetK、tetL的检出率分别为33.33%、100%、33.33%和22.22%。综上，猪链球菌2型在香格里拉市藏香猪群中流行率较高，耐药性普遍存在且多重耐药情况严重，需加强防控并制定合理的用药方案。更多还原

【Abstract】 To investigate the prevalence of Streptococcus suis type 2(SS2) in Shangri-La City, 504 serum samples and nasal swabs are collected from Tibetan pigs across 11 townships and analyzed using ELLSA, bacteria isolation and culture biochemical identification, and PCR methods. The results show an antibody positive rate of 29.76% for Streptococcus suis type 2. Eighteen strains of Streptococcus suis type 2 are isolated and identified, exhibiting varying resistance to 22 antibiotics with multidrug resistance observed in all isolates. Strong resistance is observed against tetracyclines, macrolides, and sulfonamides. These strains demonstrated strong resistance to tetracyclines, macrolides, and sulfonamides but remained sensitive to cephalosporins, penicillins, and fluoroquinolones. Resistance gene detection reveals that the prevalence rates of macrolide resistance genes mefA, msrD, ermA, and ermBwere 44.44%, 16.67%, 100%, and 22.22%, respectively. For tetracycline resistance genes, the detection rates of tetM, tetO, tetK, and tetL are 33.33%, 100%, 33.33%, and 22.22%, respectively. In conclusion, Streptococcus suis type 2 shows high prevalence in Tibetan fragrant pig populations in Shangri-La City, with widespread antibiotic resistance and severe multidrug resistance, indicating the need for strengthened prevention and control measures and the development of rational drug use protocols.更多还原