【摘要】 青花菜细胞核雄性不育材料Y37S是在自交系Y37中发现的一株自然突变不育株，为明确其遗传特性和花药败育原因，从形态学、细胞学、遗传及分子鉴定等方面进行研究。结果表明：Y37S不育系植株雄蕊退化，显著低于柱头，无花粉。扫描电镜观察结果显示，Y37S花药干瘪扭曲，呈现三角形，无花粉粒附着；利用荧光素双醋酸酯（fluorescein diacetate,FDA）对不同大小花蕾的小孢子活力进行检测，不育株花蕾发育至4.5～5.0 mm时，小孢子活力丧失且无绿色荧光信号。花药细胞学特征观察发现，不育株在单核小孢子期发生小孢子塌陷、绒毡层细胞延迟降解且降解不完全，影响了花药和花粉的正常功能，最终导致败育。用同系内6个可育株分别与Y37S进行杂交，其中Y37-1和Y37-3自交可育株与不育株比例为23︰7和17︰5,Y37-1和Y37-3与Y37S的杂交后代可育株与不育株比例分别为15︰14和28︰30，初步推测Y37S受一对隐性核基因控制。为验证其遗传规律，用Y37S与正常可育自交系B50杂交，F₁代全部可育，F₂群体中可育与不育株比为107︰29，测交群体中可育与不育株比为67︰69，符合孟德尔遗传定律，证明Y37S是由一对隐性基因控制的细胞核雄性不育系。利用已经鉴定的白菜和甘蓝单隐性核不育基因连锁的分子标记对测交群体中的不育株和可育株进行扩增，未扩增出差异条带，推测控制不育系Y37S育性的基因可能为新的隐性核不育基因。更多还原

【Abstract】 The nuclear male sterile material Y37S of broccoli is a naturally mutated sterile strain discovered in the inbred line Y37. To clarify its genetic characteristics and the causes of anther failure,studies were conducted from aspects such as morphology,cytology,genetics and molecular identification.The results showed that the stamens of the Y37S sterile line plants were degenerated,significantly lower than the stigma,and there was no pollen. The results of the scanning electron microscope showed that the anthers of Y37S were shriveled and twisted,presenting a triangular shape,with no pollen grains attached.The microspore viability of flower buds of different sizes was detected by fluorescein diacetate（FDA）.When the flower buds of sterile plants developed to 4.5–5.0 mm,the microspore viability was lost and there was no green fluorescence signal. Observation of the cytological characteristics of anthers revealed that in sterile plants,microspore collapse occurred during the mononuclear microspore stage,and the degradation of felt-like layer cells was delayed and incomplete,which affected the normal functions of anthers and pollen,ultimately leading to infertility. Six fertile strains within the same line were crossed with Y37S respectively. Among them,the ratios of self-pollinated fertile strains to sterile strains of Y37-1 and Y37-3 were 23︰7 and 17︰5,respectively,and the ratios of fertile strains to sterile strains of the hybrid offspring of Y37-1 and Y37-3 with Y37S were 15 ︰ 14 and 28 ︰ 30, respectively. It is preliminarily speculated that Y37S is controlled by a pair of recessive nuclear genes. To verify its genetic pattern,Y37S was crossed with the normal fertile inbred line B50. All F₁ generations were fertile. The ratio of fertile to sterile lines in the F₂ population was 107︰ 29,and in the test cross population, it was 67︰ 69. This conforms to Mendel’s law of inheritance and proves that Y37S is a nuclear male sterile line controlled by a pair of recessive genes. The sterile and fertile plants in the test population were amplified using the molecular markers of the single recessive nuclear sterility gene linkage of Chinese cabbage and cabbage that have been identified. No differential bands were amplified,suggesting that the gene controlling the fertility of the sterile line Y37S might be a new recessive nuclear sterility gene.更多还原