【摘要】 目的 建立同时测定紫杉醇（PTX）和顺铂（DDP）血药浓度的高效液相色谱（HPLC）法。方法 色谱柱为ODS-B C₁₈柱（250 mm×4.6 mm,5??m），流动相为甲醇-水（70∶30,V/V ），流速为1.0 mL/min，柱温为30℃，进样量为10??L，检测波长228 nm（PTX）和254 nm（DDP）。结果 PTX和DDP衍生物[Pt-二乙基二硫代氨基甲酸钠（DDTC）₂]的保留时间分别为8.84 min和12.5 min；质量浓度分别在1.0～5.0??g/mL和2.0～16.0??g/mL范围内与峰面积线性关系良好；日内和日间精密度的RSD均<10%；稳定性试验结果显示室温避光条件下质量浓度波动范围为10%～12%；冷冻条件下质量浓度波动范围为2%～4%；回收率分别为98.6%～99.4%,97.6%～98.4%（RSD均<3%）。结论 所建方法简便、准确、灵敏，结果可靠，可用于人血浆中PTX和DDP血药浓度的监测。更多还原

【Abstract】 Objective To establish a high-performance liquid chromatography（HPLC） method for the simultaneous determination of paclitaxel（PTX） and cisplatin（DDP） blood drug concentrations.Methods The chromatographic column was an ODS-B C₁₈column（250 mm × 4. 6 mm,5 ??m）,the mobile phase was methanol-water（70∶30,V/V）,the flow rate was 1. 0 mL/min,the column temperature was 30 ℃,the injection volume was 10 ??L,and the detection wavelengths were 228 nm（PTX） and 254 nm（DDP）.Results The retention times of PTX and DDP derivative [Pt-diethyldithiocarbamate（DDTC）2] were 8. 84 min and12. 5 min,respectively;the linear ranges were 1. 0-5. 0 ??g/mL and 2. 0-16. 0 ??g/mL,respectively;the RSDs of intra-day and inter-day precision were both lower than 10%;the stability test results showed that under room temperature and lightprotected conditions,the mass concentration fluctuation range was 10%-12%;under frozen conditions,the mass concentration fluctuation range was 2%-4%;the recoveries were 98. 6%-99. 4% and 97. 6%-98. 4%,respectively（RSDs lower than 3%）.Conclusion The established method is simple,accurate,sensitive,and reliable,which can be used for monitoring the blood drug concentrations of PTX and DDP in human plasma.更多还原