【摘要】 目的：建立了基于QuEChERS前处理技术同时测定小鼠血中5种尼秦类物质的超高效液相色谱-串联质谱法（UPLC-MS/MS）。方法：样本采用甲醇：乙腈（1∶3，体积比）提取，MgSO₄脱水，以XSelect HSS T₃色谱柱（2.1 mm×100 mm）进行色谱分离，正离子多反应监测（MRM）模式检测，以甲醇-0.1%甲酸水为流动相进行梯度洗脱。结果：血中5种尼秦类物质在0.1～100 ng·m L^-1范围内线性关系良好，相关系数均> 0.999 0。方法检出限为0.4～2.0 pg·m L^-1，定量下限为1.3～6.3 pg·m L^-1，加标回收率（n=6）为89.4%～110%，相对标准偏差（RSD）为3.9%～6.3%。结论：该方法具有灵敏、准确、操作性强的优点，可用于血液中5种尼秦类物质的检验，为涉毒案件提供科学依据。更多还原

【Abstract】 Objective: A method was established for the simultaneous determination of five nitazenes in the blood of mice using ultra-high performance liquid chromatography-tandem mass spectrometry（UPLC-MS/MS） based on Qu ECh ERS pretreatment technology. Methods: The samples were extracted with a mixture of methanol-acetonitrile（1 ∶ 3, volume ratio） and dehydrated with MgSO₄. The samples were separated on an XSelect HSS T₃ column（2.1 mm×100 mm） by positive ion scanning in multiple reaction monitoring（MRM） mode, with gradient elution using a mobile phase composed of methanol-0.1% formic acid aqueous solution. Results: Five nitazenes exhibited good linearities within the concentration range of 0.1-100 ng · mL^-1, with correlation coefficients all exceeding 0.999 0. The limits of detection（LOD） of five nitazenes were within the range of 0.4-2.0 pg · m L^-1, and the limits of quantitation（LOQ） were within the range of 1.3-6.3 pg · m L^-1. The spiked recovery rates（n=6） ranged from 89.4% to 110%, with relative standard deviations（RSD） of 3.9%-6.3%. Conclusion: This method offers advantages including high sensitivity, accuracy, and strong operability. It can be effectively applied to detect five nitazenes in blood samples, providing a scientific basis for drug-related cases.更多还原