【摘要】 目的:通过谱-效相关分析，针对当归补血汤的“补血”功效，阐明其药效物质基础。方法:运用液质联用(LC-QE/MS)技术对当归补血汤进行化学成分定性分析；建立当归补血汤超高效液相(UPLC)指纹图谱，对30批次自制当归补血汤的共有峰进行定量分析；建立苯肼诱导的斑马鱼贫血模型，应用谱-效相关分析模式，将30批次当归补血汤的药效数据与指纹图谱数据关联分析，筛选其药效物质基础。结果:当归补血汤原型成分定性分析共鉴定出90个化合物；成功建立当归补血汤UPLC指纹图谱及斑马鱼贫血模型，30批次当归补血汤的药效数据与指纹图谱数据关联分析得到当归补血汤的药效物质有4-胍基丁酸(黄芪)、异亮氨酸(酒当归)、月桂酸(酒当归)、阿魏酸(酒当归、黄芪)、6-甲氧基-2H-色烯-2-酮(酒当归、黄芪)、毛蕊异黄酮葡萄糖苷(酒当归、黄芪)、未知峰8。结论:本研究通过当归补血汤的原型化学成分定性定量分析并结合谱-效相关研究方法，筛选了当归补血汤的药效物质基础，为该经典名方制剂的临床使用和大品种二次开发提供了科学支撑。更多还原

【Abstract】 Objective: To elucidate the pharmacological material basis of Danggui Buxue Decoction for its “blood-tonifying” effect through spectrum-effect relationship analysis. Methods: The chemical constituents of Danggui Buxue Decoction were qualitatively analyzed using liquid chromatography-quadrupole-orbitrap mass spectrometry(LC-QE/MS). An ultra-performance liquid chromatography(UPLC) fingerprint of Danggui Buxue Decoction was established, and the common peaks of 30 batches of self-made Danggui Buxue Decoction were quantitatively analyzed. A zebrafish anemia model induced by phenylhydrazine was established, and the spectrum-effect relationship analysis method was applied to correlate the pharmacological data of 30 batches of Danggui Buxue Decoction with the fingerprint data to screen for its pharmacological material basis. Results: A total of 90 prototype components were identified in the qualitative analysis of Danggui Buxue Decoction. The UPLC fingerprint of Danggui Buxue Decoction and the zebrafish anemia model were successfully established. The spectrum-effect relationship analysis of the pharmacological data and fingerprint data of 30 batches of Danggui Buxue Decoction revealed that the pharmacological materials of Danggui Buxue Decoction included 4-guanidinobutanoic acid(from Astragalus), isoleucine(from wine-prepared Angelica), lauric acid(from wine-prepare Angelica), ferulic acid(from both wine-prepared Angelica and Astragalus), 6-methoxy-2H-chromen-2-one(from both wine-prepared Angelica and Astragalus), sophoraisoflavone glucoside(from both wine-prepared Angelica and Astragalus), and an unknown peak 8. Conclusion: This study screened the pharmacological material basis of Danggui Buxue Decoction through qualitative and quantitative analysis of its prototype chemical constituents combined with spectrum-effect relationship analysis, providing scientific support for the clinical application and secondary development of this classic prescription.更多还原